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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(7): 1420-1425
DOI: https://doi.org/10.20546/ijcmas.2019.807.169


Comparison of KOH, Calcofluor White and Fungal Culture in the Diagnosis of Onychomycosis
R. Shakthi* and D. Venkatesha
Department of Microbiology, Adichunchanagiri Institute of Medical Sciences, B.G. Nagara - 571448, Mandya. India
*Corresponding author
Abstract:

Onychomycosis is a fungal infection of the finger or toe nail caused by various fungal agents like dermatophytes, non dermatophytes moulds and yeasts. It is necessary to diagnose the infection with proper laboratory evidences before treating them with antifungal drugs, which require prolonged duration of treatment and may have adverse side effects. This study involves comparison of standard laboratory tests in the diagnosis of onychomycosis, namely, potassium hydroxide mount (KOH mount), Calcofluor white staining and fungal culture of the nail clippings. A total of 100 patients with clinically suspected onychomycosis were selected. Nail scrapings and clippings were subjected to KOH mount, Calcofluor staining for direct microscopic examination and culture using Sabouraud’s dextrose agar. Out of the 100 patients, direct microscopy with KOH mount, calcofluor mount and fungal culture showed positive results in 69(69%), 80(80%), 54(54%) respectively. Out of the 54 culture positive 29 were Dermatophyte, 20 were non dermatophytes mould (Aspergillus) and 5 were Candida species. Culture was taken as the gold standard and was compared with KOH mount and calcofluor white microscopy. The sensitivity and specificity of KOH microscopy was 83.05% and 56.09% respectively, whereas calcofluor white microscopy showed 92.3% and 54.2% respectively. Calcofluor white is the best method to detect fungal agents from clinically suspected onychomycosis cases with high sensitivity. It is easy to perform, rapid, and give significantly higher rates of detection of onychomycosis compared to the KOH mount and fungal culture.


Keywords: Onychomycosis, KOH mount, Calcofluor white, fungal culture
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How to cite this article:

Shakthi, R. and Venkatesha, D. 2019. Comparison of KOH, Calcofluor White and Fungal Culture in the Diagnosis of Onychomycosis.Int.J.Curr.Microbiol.App.Sci. 8(7): 1420-1425. doi: https://doi.org/10.20546/ijcmas.2019.807.169