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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(1): 859-865
DOI: http://dx.doi.org/10.20546/ijcmas.2017.601.101


Anti Biofilm and Anti Plasmid Activites of Syzygium aromaticum and Kaempheria galanga against Pseudomonas aeruginosa
Neelima Christopher1, Rajesh Ramachandran2* and Khaleel Ahamed Thaha3
 1Student, SBST, VIT University,Vellore, India
2Director, Biogenix Research Center, Trivandrum, India
3Senior Resident, Dept. of Conservative Dentistry & Endodontics, Govt.Dental College, Trivandrum, India
*Corresponding author
Abstract:

Objective of the research was to validate the potential of Syzygium aromaticum and Kaempheria galanga against biofilm formation and plasmid borne drug resistance exhibited by Pseudomonas spp. Antimicrobial property of S.aromaticum and K.galanga on P.aeruginosa culture was assessed using the agar well diffusion method. Crystal violet assay was employed to determine the percentage inhibition of P.aeruginosa biofilm by the extracts following generation of biofilm on multiwell plates. The decrease in Pseudomonas viability in an established biofilm after treatment was determined by standard MTT reduction assay. Fluorescent microscopy following staining with Ethidium bromide and Acridine orange was done to distinguish the dead and live cells in the biofilm. Finally, antiplasmid activity was established by agarose gel electrophoresis. The extracts were found to have potent antibacterial activity and inhibited biofilm attachment to polymeric surfaces. They further exhibited a significant decrease in biofilm bacterial cell viability when determined by MTT assay and fluorescent staining. S aromaticum effectively removed plasmids from Pseudomonas spp which can be due to their potential curing efficiency. The results obtained from the study validate the antibacterial and anti plasmid potential of extracts from Syzygium aromaticum and Kaempheria galanga against P.aeruginosa biofilm. The combination may be recommended as a potential drug to prevent biofilm formation and plasmid borne drug resistance.


Keywords: Kaempheria galangal,Anti Biofilm and Anti Plasmid Pseudomonas aeruginosa
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How to cite this article:

Neelima Christopher, Rajesh Ramachandran and Khaleel Ahamed Thaha. 2017. Anti Biofilm and Anti Plasmid Activites of Syzygium aromaticum and Kaempheria galanga against Pseudomonas aeruginosa.Int.J.Curr.Microbiol.App.Sci. 6(1): 859-865. doi: http://dx.doi.org/10.20546/ijcmas.2017.601.101