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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(7): 1226-1233
DOI: https://doi.org/10.20546/ijcmas.2019.807.146


Evaluation of Sterilant Effect on In-vitro Culture Establishment in Sugarcane Variety Co 0118
Devendra Kumar1*, Rakesh Singh Sengar1, Manoj Kumar Yadav1, Pooranchand2, Gopal Singh3 and Shalani Gupta4
1Department of Agricultural Biotechnology,
2Department of Genetics and Plant Breeding,
3Department of Plant Pathology,
4Department of Molecular Biology and Genetic Engineering, College of Biotechnology, S.V.P.U.A. & T., Meerut- 250110 (U.P.), India
*Corresponding author
Abstract:

In-vitro propagation is widely used as reliable alternative for true-to-type and quality seed production in sugarcane. The main obstacle under in-vitro propagation is growth of contaminants, resulting in failure of culture establishment that can be minimized by effective surface sterilization method. The sterilization process varied species to species and explants type thus, present study was designed to evaluate the effects of various sterilants on in-vitro culture establishment of sugarcane variety Co 0118 through meristem shoot tip. The explants were collected and treated with different concentrations of sterilants viz., BavistinTM (0.01-0.4%), HgCl2 (0.01-0.4%), NaOCl (2-10%) and EtOH (60-100%). The treated explants were inoculated on semi solid MS media supplemented with 0.5 mg/L each of BAP and Kn. The results obtained under study showed significant differences in their activity. The optimum culture survival percent and lower contamination with reduced necrosis was to be found when explants were treated with the combination of 0.1% Bavistin for 10 min followed by 0.1% HgCl2 for 5 min, 6% NaOCl for 10 min and 70% EtOH for 1 min. This sterilization protocol will be contributive in efficient surface sterilization for in-vitro propagation of sugarcane though meristem shoot tip.


Keywords: Sterilization, Culture, Contamination, Meristem shoot, Sterilants
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How to cite this article:

Devendra Kumar, Rakesh Singh Sengar, Manoj Kumar Yadav, Pooranchand, Gopal Singh and Shalani Gupta. 2019. Evaluation of Sterilant Effect on In-vitro Culture Establishment in Sugarcane Variety Co 0118.Int.J.Curr.Microbiol.App.Sci. 8(7): 1226-1233. doi: https://doi.org/10.20546/ijcmas.2019.807.146