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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(7): 1217-1225
DOI: https://doi.org/10.20546/ijcmas.2019.807.145


Effect of Different Plant Growth Regulators on In-vitro Callus Induction in Carica papaya (cv. Pusa Nanha)
Nisha Malik1*, Rakesh Singh Sengar1, Manoj Kumar Yadav1, Shiv Kumar Singh2, Gopal Singh3 and Mukesh Kumar4
1Department of Agricultural Biotechnology,
2Department of Genetics and Plant Breeding,
3Department of Plant Pathology,
4Department of Horticulture, College of Horticulture, S.V.P.U.A. &T., Meerut- 250110 (U.P.), India
*Corresponding author
Abstract:

The present investigation was conducted to optimize an efficient callus induction protocol in Carica papaya (cv. Pusa Nanha). Two different types of explants were collected from field grown papaya plants under study viz., leaf and petiole. The explants were surface sterilized and excised into 1-2 cm diameter and inoculated on basal MS medium supplemented with various concentrations of NAA, Kinetin and TDZ. The cultures were kept in dark inside the culture room at 25 ± 2°C and humidity 60-65%. The results obtained under study indicate that media containing only NAA and Kinetin results in less percent of callus induction in both explants (11.33 to 30% in leaf and 3.66 to 7% in petiole) whereas earliest and significantly (p<0.05) higher callus induction was obtained in media containing 3.0 mg/L NAA + 0.5 mg/L Kin and 1.5 µM TDZ. Callus produced from leaf was soft and spongy and white in colour while petiole produced hard and friable callus appeared dark in colour. The findings of study suggesting leaf as the best explant and TDZ as an effective growth regulator for callus production in papaya. This protocol for callus induction may also help in propagating papaya plants via somatic embryogenesis, organogenesis and transformation studies.


Keywords: Papaya, Callus, NAA, Kinetin, TDZ
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How to cite this article:

Nisha Malik, Rakesh Singh Sengar, Manoj Kumar Yadav, Shiv Kumar Singh, Gopal Singh and Mukesh Kumar. 2019. Effect of Different Plant Growth Regulators on In-vitro Callus Induction in Carica papaya (cv. Pusa Nanha).Int.J.Curr.Microbiol.App.Sci. 8(7): 1217-1225. doi: https://doi.org/10.20546/ijcmas.2019.807.145