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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(1): 834-843
DOI: http://dx.doi.org/10.20546/ijcmas.2017.601.098


Influence of Silver and Copper Nanoparticles on Physiological Characteristics of Phaseolus vulgaris L. in vitro and in vivo
Harir S.  Mustafa1, Asma G. Oraibi2*, Kadhim M. Ibrahim2 and Nabeel K. Ibrahim2
1College of Science, Department of Biotechnology, Al-Nahrain University, Baghdad, Iraq
2College of Biotechnology, Department of Agricultural Biotechnology,Al-Nahrain University, Baghdad, Iraq
*Corresponding author
Abstract:

Phaseolus vulgaris seeds were surface sterilized using ethanol 90%, soaked in a sterile distilled water for 60min, then soaked in copper and silver nanoparticles at different concentrations (25, 50 and 100mg/ml) for 15 and 30min. The effect of nanoparticles on the plant physiological characteristics in vivo was detected. Callus was initiated from treated and non–treated seed embryos, then embryos were maintained and transferred to callus induction medium Murashige and Skoog (MS) medium supplemented with combinations of plant growth regulators including Dichlorophynoxyacetic acid (2,4-D) (2.5mg/l), Benzyl adenine (BA) (0.5mg/l). The effects of nanoparticles on %callus induction, callus fresh weight and callus dry weight were observed. Results indicated that the effective concentration of nanoparticles that affect the physiological parameters of treated plant, % callus induction, callus fresh and dry weight was 50mg/ml sliver nanoparticles at 30min.


Keywords: Phaseolus vulgaris L., silver nanoparticles, copper nanoparticles. 
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How to cite this article:

Harir S.  Mustafa, Asma G. Oraibi, Kadhim M. Ibrahim and Nabeel K. Ibrahim. 2017. Influence of Silver and Copper Nanoparticles on Physiological Characteristics of Phaseolus vulgaris L. in vitro and in vivo.Int.J.Curr.Microbiol.App.Sci. 6(1): 834-843. doi: http://dx.doi.org/10.20546/ijcmas.2017.601.098