Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(7): 1345-1350
DOI: https://doi.org/10.20546/ijcmas.2019.807.160


In-vitro Multiple Shoots Production from Cormel Shoot Buds in Gladiolus (Gladiolus hybrida)
Parul Devi, Pushpendra Kumar*, R.S. Sengar, M.K. Yadav, Mukesh Kumar, S.K. Singh and Shilpy Singh
Department of Agricultural Biotechnology, Sardar Vallabhbai Patel University of Agriculture and Technology, Meerut, U.P. - 250110, India
*Corresponding author
Abstract:

Gladiolus (Gladiolus hybrida) is an important ornamental plant cultivated world over for its attractive spikes. The commercial cultivation of Gladiolus is based on natural multiplication of corms and cormels. In vitro propagation techniques, is superior to conventional propagation and produces disease free plants in huge quantities. In vitro regeneration of gladiolus cultivars, Sylvia, White Prosperity and Amsterdam was achieved using shoot bud of cormels as an explant. Sylvia variety recorded the best for callus induction and regeneration than other two varieties. The concentration of plant growth regulators affects the regeneration response differently. The highest callus induction rate of 76% explants was recorded on Murashige and Skoog (MS) medium supplemented with 2 mgl-1 2,4-D (2,4-Dichlorophenoxyacetic acid), after 28 days. MS medium supplemented with BAP at 2.0 mgl-1 exhibited higher shoot proliferating efficiency, i.e shoots per explant in Sylvia variety.


Keywords: Gladiolus hybrida, in vitro regeneration, Growth regulators, Dichlorophenoxy acetic acid, Benzylaminopurine
Download this article as Download

How to cite this article:

Parul Devi, Pushpendra Kumar, R.S. Sengar, M.K. Yadav, Mukesh Kumar, S.K. Singh and Shilpy Singh. 2019. In-vitro Multiple Shoots Production from Cormel Shoot Buds in Gladiolus (Gladiolus hybrida).Int.J.Curr.Microbiol.App.Sci. 8(7): 1345-1350. doi: https://doi.org/10.20546/ijcmas.2019.807.160