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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(1): 811-818
DOI: http://dx.doi.org/10.20546/ijcmas.2017.601.096


In Vivo Studies of Antibacterial Activity of Gongronema latifolium and Antibiotics on Staphylococcus aureus Infected Albino Rats
K.H. Enyi-Idoh1*, C.C. Njoku1, V.D. Idim2, I.U. Bassey1 and S. U. Egeonu3
1Department of Microbiology, Faculty of Biological science, University of Calabar Calabar, Nigeria
2Department of Chemical Sciences, Cross River University of Technology, Calabar, Cross River State, Nigeria
3Federal Medical Center, Jalingo, Taraba State, Nigeria
*Corresponding author
Abstract:

Many plants in Africa are medicinal. The use of medicinal natural plants predates the establishment of antibiotics and other modern drugs into the African continent. The antibacterial activity of Gongronema latifolium and a synthetic antibiotic (Rocephin) on a clinical isolate of Staphylococcus aureus was evaluated in vivo using albino Wister rats. Five (5) adult female rats with weight ranging from 120 – 180 kg were purchased, housed, fed and nurtured for five days. Four (4) of the rats were inoculated with 0.1 ml 4hour growing broth culture of Staphylococcus aureus. Blood was collected from the rats prior, during and after inoculation and treatment for haematological analysis and bacteraemia. Treatment was done by administering 0.5ml of Rocephin (equivalent to 0.125mg) to rat 1 and 0.5ml of purified ethanolic plant extract (equivalent to 0.5mg) to rat 2, A combination of both preparations as above was administered to rat 3. Rat 4 was not given any treatment. Results indicated that the extract of G. latifolium leaves possesses higher antibacterial activity than the antibiotic Rocephin and the combination therapy. This result has shown the relative potency of G. latifolium against Staphylococcus aureus bacteria in vivo.


Keywords: Gongronema, Staphylococcus, albino rats, Rocephin, treatment.
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How to cite this article:

Enyi-Idoh, K.H., C.C. Njoku, V.D. Idim, I.U. Bassey and Egeonu, S. U. 2017. In Vivo Studies of Antibacterial Activity of Gongronema latifolium and Antibiotics on Staphylococcus aureus Infected Albino Rats.Int.J.Curr.Microbiol.App.Sci. 6(1): 811-818. doi: http://dx.doi.org/10.20546/ijcmas.2017.601.096