Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(7): 933-938
DOI: https://doi.org/10.20546/ijcmas.2019.807.112


Molecular Characterization of Heat Tolerance Chickpea (Cicer arietinum L.) Genotypes using Random Amplified Polymorphic DNA (RAPD) Markers
Devendra Vasht1*, S.K. Dwivedi1, Yogendra Singh2, R.K. Samaiya1,A.  Babbar2, S.K. Pooniya3 and Vinod Kumar2
1Department of Plant Physiology
2Department of Plant Breeding & Genetics
3Department of Biotechnology, Jawaharlal Nehru KrishiVishwaVidyalaya, Jabalpur (MP), India
*Corresponding author
Abstract:

Chickpea (Cicer arietinum L.) is most important pulse crop of India in term of area and production and is extensively grown from human consumption mainly in South Asia and Middle East. Chickpea (Cicer arietinum L.) is a cool season legume crop and is grown in several countries worldwide as a food source. Molecular markers and variation in genomic DNA sequences have played a major role in varietal characterization and improvement of many crop species and have contributed to our abilities to assess biodiversity, reconstruct accurate phylogenetic relationships and transferring of particular traits. The polymorphism % ranged from 33.33 % (OPX-2) to 100% (OPB-6, OPX-4, OPB-12, OPA-17, OPA-11). The dendrogram was generated and 20 genotypes were aligned in 3 groups i.e. A has 10 genotypes, B has only one genotype and C has 9 genotypes. For molecular characterization of 20 genotypes, 10 RAPD primers showing polymorphism were used.


Keywords: DNA Isolation, RAPD primers, Polymorphism
Download this article as Download

How to cite this article:

Devendra Vasht, S.K. Dwivedi, Yogendra Singh, R.K. Samaiya, A. Babbar, S.K. Pooniya and Vinod Kumar. 2019. Molecular Characterization of Heat Tolerance Chickpea (Cicer arietinum L.) Genotypes using Random Amplified Polymorphic DNA (RAPD) Markers.Int.J.Curr.Microbiol.App.Sci. 8(7): 933-938. doi: https://doi.org/10.20546/ijcmas.2019.807.112