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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(1): 798-806
DOI: http://dx.doi.org/10.20546/ijcmas.2017.601.094


Isolation and Characterization of Native Isolates of Bacillus thuringiensis from Vidarbha Region

Dipika Ashokrao Padole1*, Mangesh Pradip Moharil1, Krishnananda Pralhad Ingleand Shyamkant Munje2

1Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola,Maharashtra, India
2Regional Research Centre for Soybean, Amaravati, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Maharashtra, India
*Corresponding author
Abstract:

The present investigation was undertaken for the isolation and characterization of B. thuringiensis strains from different habitats based on the morphology, staining properties and biochemical characters. Fifty four samples including soil samples (30), phylloplane (21), insect cadaver (2) and stored grain dusts (1) were collected from cultivated and barren lands not having Bt history. Total 154 Bt isolates obtained from different sources comprising 89, 59 and 6 from soil, phylloplane and insect cadaver respectively, whereas, none of the single isolate was obtained from seed dust. The local Bt isolates showed positive morphological characters as that of standard strain Bacillus thuringiensis kurstaki HD -1 (Btk HD-1). Phase contrast microscopic evaluation showed that, more than 75% of the total 154 isolates lead significant resemblance with standard strain. Further characterization of the probable native Bt isolates was carried out biochemically with different tests such as IMViC tests and for the presence of enzymes like catalase, urease, and nitrate reductase utilization which showed that 70-80% of the total probable Bt isolates has positive characters as standard strain. Bt isolation index suggested that soil had highest background Bt population followed by insect cadaver and leaves.


Keywords: Bacillus thuringiensis, IMViC, Phase contrast microscopy, Btk HD-1 - Bacillus thuringiensis var. kurstaki HD-1, Malachite green
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How to cite this article:

Dipika Ashokrao Padole, Mangesh Pradip Moharil, Krishnananda Pralhad Ingle, Shyamkant Munje. 2017. Isolation and Characterization of Native Isolates of Bacillus thuringiensis from Vidarbha RegionInt.J.Curr.Microbiol.App.Sci. 6(1): 798-806. doi: http://dx.doi.org/10.20546/ijcmas.2017.601.094