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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(7): 456-466
DOI: https://doi.org/10.20546/ijcmas.2019.807.056


Molecular Identification and in silico Characterization of Coat Protein in Tomato Leaf Curl Virus Associated in Tomato from South Gujarat Region of India
Solanki Ravindra and R.K. Kalaria*
ASPEE Shakilam Biotechnology Institutes, Navsari Agricultural University, Surat, India
*Corresponding author
Abstract:

Tomato leaf curl virus disease (ToLCVD) is one of the major causes for highest economical loss of tomato in India. In this study, Natural incidence of ToLCV disease on tomato was observed at Gujarat, India in the March, 2019. The infected plants revealed systemic viral symptoms in the form of sever leaf curling, leaf crumple with marginal yellowing, stem warped and stunted. Molecular methods were followed to further characterized coat protein (CP) gene of tomato leaf curl virus using specified primers. The purified PCR product (~800 bp) was sequenced and further analysed showed that the virus had 99.75% sequence identities and closest phylogenetic relationships with various isolates of Tomato leaf curl New Delhi virus, therefore name as Tomato leaf curl virus isolate NAU Gujarat coat protein (AV1) gene and submitted to NCBI (MK955892.1). ToLCV coat-protein sequence was computationally analysed to study evolutionally classification and further modelled to predict structure using swiss model that further validated using Ramachadran plot showing 89.10% amino acid in most favourable region. The present studied may be useful for development of diseases resistance approach for various crops against begomoviruses to minimize the yield losses.


Keywords: Begomovirus, Tomato leaf curl virus, PCR, AV1 gene, Whiteflies and Phylogenetic relationship
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How to cite this article:

Solanki Ravindra and Kalaria, R.K. 2019. Molecular Identification and in silico Characterization of Coat Protein in Tomato Leaf Curl Virus Associated in Tomato from South Gujarat Region of India.Int.J.Curr.Microbiol.App.Sci. 8(7): 456-466. doi: https://doi.org/10.20546/ijcmas.2019.807.056