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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(7): 431-437
DOI: https://doi.org/10.20546/ijcmas.2019.807.053


Antimicrobial Activity of Azadirachta indica (Neem) Leaf Extract on Some Bacteria
Ugwu Celestina Chibuzo*
Enugu State University of Science and Technology (ESUT) Enugu, Enugu State, Nigeria
*Corresponding author
Abstract:

The antimicrobial activity of Azadirachta indica leaf extract (Neem leaf) was carried out on Staphylococcus aureus, Pseudomonas aeruginosa (ATCC 27853) and Escherichia coli using the agar well diffusion method. Ethanolic, aqueous and methanolic extracts of the plant were used at varying concentrations of 200 mg/ml, 100 mg/ml, 50 mg/ml, 25 mg/ml, 12.5 mg/ml, 6.25 mg/ml, 3.125 mg/ml and 1.56 mg/ml respectively. The three extracts were found to show a very high antimicrobial activity though the ethanolic extract possessed more activity than the methanolic and aqueous extracts. The minimum inhibitory concentration (MIC) was found to range from 1.56 mg/ml to 6.125for the three extracts respectively. When compared with standard antibiotics, the ethanolic and methanolic extracts showed maximum inhibition on these organisms. The phytochemical analyses revealed the presence of alkaloids, saponins, tannins, glycosides, steroids, terpenoids, and reducing sugars. Leaf extract of Azadirachta indica is therefore recommended for the treatment of human infections associated with these organisms.


Keywords: Azadirachta indica, Staphylococcus aureus, Escherichia coli, Phytochemical constituents, Inhibition
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How to cite this article:

Ugwu Celestina Chibuzo. 2019. Antimicrobial Activity of Azadirachta indica (Neem) Leaf Extract on Some Bacteria.Int.J.Curr.Microbiol.App.Sci. 8(7): 431-437. doi: https://doi.org/10.20546/ijcmas.2019.807.053