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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:7, July, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(7): 132-141
DOI: https://doi.org/10.20546/ijcmas.2019.807.017


Evaluation of Skin Expression Profiles in Cattle of Bos indicus and Bos taurus Lineage Following Rhipicephalus annulatus Infestation and Molecular Characterisation of S100A8 Gene
Elizabeth Kurian1*, C.N. Dinesh1, Reghu Ravindran2, Naicy Thomas1, Jain Jose1,K. Raji3, K.A. Bindu1 and T.V. Aravindakshan1
1Department of Animal Breeding and Genetics,
2Department of Veterinary Parasitology,
3Department of Veterinary Physiology, Kerala Veterinary and Animal Sciences University, Kerala, India
*Corresponding author
Abstract:

In tropical countries, a huge drain in profitability of animal production sector is attributed to tick and tick- borne diseases (TTBD). Traditional tick control measures are riddled by development of acaricide resistance in ticks and possibility of environmental pollution. Identifying and propagating naturally resistant breeds of cattle in endemic regions is a more efficient method in the long run. Marker assisted selection (MAS) which applies genomic information along with conventional selection in cattle breeding is a probable solution. S100A8 encodes a protein with profound pro-inflammatory, antimicrobial and antifungal activities in skin. Differential expression of the gene in the wake of Rhipicephalus annulatus infestation in Vechur a native breed of cattle in comparison to crossbred Holstein Friesian (CBHF) was studied using quantitative polymerase chain reaction (qPCR). The exons and introns of S100A8 gene were characterized in three genetic groups, Vechur, Kasargode (Bos indicus) and crossbred Holstein Friesian (Bos taurus), to identify the single nucleotide polymorphism (SNPs) present in among different populations.


Keywords: Tick resistance, S100A8 expression, Calprotectin, Inflammation, SNP

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How to cite this article:

Elizabeth Kurian, C.N. Dinesh, Reghu Ravindran, Naicy Thomas, Jain Jose, K. Raji, K.A. Bindu and Aravindakshan, T.V. 2019. Evaluation of Skin Expression Profiles in Cattle of Bos indicus and Bos taurus Lineage Following Rhipicephalus annulatus Infestation and Molecular Characterisation of S100A8 Gene.Int.J.Curr.Microbiol.App.Sci. 8(7): 132-141. doi: https://doi.org/10.20546/ijcmas.2019.807.017
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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