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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(7): 23-31
DOI: https://doi.org/10.20546/ijcmas.2019.807.004


Molecular Detection of Virulence Associated Genes in Salmonella Serovars Isolated From Raw Pork of Aizawl and Imphal
M. Das1, E. Motina1, D. Deka1, T.K. Dutta2, P. Roychowdhury2, A. De3 and S. Chakraborty2*
1Department of Veterinary Public Health & Epidemiology, College of Veterinary Sciences and animal Husbandry, Central agricultural University, Selesih, Aizawl, Mizoram - 796014, India
2Department of Veterinary Microbiology, College of Veterinary Sciences and animal Husbandry, Central agricultural University, Selesih, Aizawl, Mizoram - 796014, India
3Department of Veterinary Physiology and Biochemistry, CVSc & AH, R.K. Nagar, West Tripura – 799008, India
*Corresponding author
Abstract:

The study was aimed to detect virulence associated genes of Salmonella serovars, which were isolated from raw pork samples of unorganised butcher shops of Aizawl and Imphal. A total of 5 Salmonella isolates (Aizawl=2, Imphal=3), belonging to Salmonella vircho (n=4), and Salmonella typhimurium (n=1), were obtained from 200 samples (100= Aizawl and 100= Imphal) and screened for five virulence associated genes, namely invA, stn, pefA, sefC, spvC by Polymerase Chain Reaction (PCR) technique. All Salmonella serovars detected positive for invA and stn genes, spvC and pefA gene was found positive in Salmonella typhimurium and one Salmonella Vircho of Aizawl origin (overall 40%). sefA was absent in all isolates. All the Salmonella serovars possessed invasive gene (invA) and enterotoxin gene (stn), which make them capable for producing gastroenteritis in human. Presence of Salmonella typhimurium positive with 4 virulence associated genes in Aizawl is a matter of worry from hygienic point of view.


Keywords: Salmonella, Virulence, PCR, Pork, Imphal, Aizawl
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How to cite this article:

Das, M., E. Motina, D. Deka, T.K. Dutta, P. Roychowdhury, A. De and Chakraborty, S. 2019. Molecular Detection of Virulence Associated Genes in Salmonella Serovars Isolated From Raw Pork of Aizawl and Imphal.Int.J.Curr.Microbiol.App.Sci. 8(7): 23-31. doi: https://doi.org/10.20546/ijcmas.2019.807.004