Banana variety Nanjanagudu rasabale has been found growing in parts of Mysuru district, known for its unique aroma, flavour, taste and shelf life but unfortunately deemed as an endangered variety. Having given GI protection hope for bringing this variety back to field by producing disease free tissue culture plants demanded development of an efficient protocol for in vitro regeneration. In the present investigation, both citric acid and ascorbic acid found effective in inhibiting browning of shoot tip explant due to phenolic compounds. Each of the chemical sterilants was effective in reducing microbial contamination when they were used in sequence one after the other.MS media supplemented with BAP at 3.0 mg/l took least number of days for shoot regeneration and favoured better shoot production with maximum number of leaves per shoot and shoot length. Addition of 25mg/l adenine sulphate and 2-3 ml of Aonla juice proliferated maximum number of shoots during multiplication. MS media at half strength provided with activated charcoal and IBA at 2 mg/l was effective in producing better roots from in vitro grown shoots.

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