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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 3160-3171
DOI: https://doi.org/10.20546/ijcmas.2019.806.378


Computational Interaction Analysis of Cyprinus carpio Trypsin1 and Kunitz Type Soybean Protease Inhibitor
Gulshan Kumar*, Munish Kumar, Gyandeep Gupta and Ranjeeta Kumari
Division of Genetics and Biotechnology, ICAR- Central Institute of Fisheries Education, Versova - 400 061, Mumbai, India
*Corresponding author
Abstract:

Common carp (Cyprinus carpio) is highly important culturable species in freshwater and cultured throughout the globe. Its farming require formulated feed with balance protein and energy. Most of the time fish meal is used as a protein source, but it is not acceptable from sustainability point of view. The replacement of fish meal by plant based protein source is advised. The limitation of plant based ingredients like soybean meal is the presence of anti-digestive factors in the form of several protease inhibitors. Kunitz type soybean protease inhibitor (SPI) is the most abundant protease inhibitor in soybean meal and it reduces the digestibility of feed. The data regarding molecular interaction and binding affinity of SPI with proteases is available in mammalian organism like pig but in fishes it has not been studied in detail. So the present study was conducted to gain insight into the protein-protein interaction and binding affinity of common carp trypsin (ccTrypsin1) and SPI using computational modelling. The sequence and 3D structure of ccTrypsin1 is highly similar to its mammalian counterpart pig. The protein-protein interaction analysis using knowledge based docking approach showed that the interaction was more or less similar like porcine trysin with SPI. The predicted binding affinity (-9.2 kcal/mol) and dissociation constant (1.9E-0.7) showed strong interaction between the interactor governed by strong attractive forces like electrostatic forces.


Keywords: Cyprinus carpio, Aquaculture, Protease inhibitor, Trypsin, Soybean meal, computational modelling
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How to cite this article:

Gulshan Kumar, Munish Kumar, Gyandeep Gupta and Ranjeeta Kumari. 2019. Computational Interaction Analysis of Cyprinus carpio Trypsin1 and Kunitz Type Soybean Protease Inhibitor.Int.J.Curr.Microbiol.App.Sci. 8(6): 3160-3171. doi: https://doi.org/10.20546/ijcmas.2019.806.378