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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Index Copernicus ICV 2017: 100.00
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Int.J.Curr.Microbiol.App.Sci.2019.8(6): 3071-3081
DOI: https://doi.org/10.20546/ijcmas.2019.806.366


Analysis of Allelic Differentiation and Prediction of Suitable Parents among Brassica juncea L. Genotypes using Microsatellite Markers
Mir Ahmad Habib, Manmohan Sharma, Punya*, R.K. Salgotra and Usha Kiran
School of Biotechnology, SKUAST-J, Chatha, Jammu (J&K), India
*Corresponding author
Abstract:

Information on allelic differentiation is very important to elucidate the nature and extent of divergence among Brassica juncea genotypes. In present study 20 Brassica juncea L. genotypes generated 79 polymorphic alleles from 15 SSR primer pairs with an average of 5.26 alleles per locus. The polymorphism percentage was recorded to be the maximum in the case of the primer Ni4- D04 (80%) and the minimum in the case of 3 different primers namely A06-20686249, Ni3-D03 and A05-20242013 (33.3%). The polymorphism information content (PIC) values ranged from 0.41 (A01-13393871) to 0.94 (A05-25290881), reflecting the presence of high allelic variation. Considering the number of alleles generated by different primer pairs in conjunction with the level of polymorphism detected in the present study, the primers A04-9627743 and A04-15440685 appeared to be more informative primers. The magnitude of similarity coefficient between RH749 and Kranti (0.368) was found to be the maximum amongst pair-wise combinations of genotypes under evaluation in the present study and considered as most similar genotypes. The dendrogram generated with UPGMA cluster analysis revealed six major clusters. Genotype combinations VNIIK 405 and J/817/2, Miike Akachirimen and RH749, and VNIIK 405 and RH749 exhibited significant genetic diversity and were consider as suitable parent combinations for breeding programmes. The genotype Vardan was found to be distinct from all other groups. SSR markers utilized in the present study proved efficient in discrimination and unambiguous classification of genotypes and for identifying diverse parental combinations.


Keywords: Brassica juncea L. genotypes, Alleles, SSRs, Genetic variability
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How to cite this article:

Ahmad Habib, Mir, Manmohan Sharma, Punya, R.K. Salgotra and Usha Kiran. 2019. Analysis of Allelic Differentiation and Prediction of Suitable Parents among Brassica juncea L. Genotypes using Microsatellite Markers.Int.J.Curr.Microbiol.App.Sci. 8(6): 3071-3081. doi: https://doi.org/10.20546/ijcmas.2019.806.366