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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 2762-2769
DOI: https://doi.org/10.20546/ijcmas.2019.806.333


Morphological and Molecular Characterization of Marssonina coronaria (Ellis & J.J. Davis) J.J. Davis Causing Premature Leaf Fall
S. Phurailatpam*, J.N. Sharma, A. Brakta, Sushma Sharma and Shweta Sharma
Department of Plant Pathology, College of Horticulture, Dr. Y.S. Parmar University of Horticulture and Forestry, Solan173 230, Himachal Pradesh, India
*Corresponding author
Abstract:

Marssonina leaf blotch caused by the fungus Marssonina coronaria (Ellis & J.J. Davis) J.J. Davis causing premature leaf fall is one of the major economic constraint which hampers apple production in India. Seven isolates of Marssonina coronaria were isolated and evaluated for their morphological and molecular characterization using ITS primers. All the seven isolates were identified based on the morphological characteristics, and produced brown to black colonies without aerial mycelium, formed a wrinkled surface, on PDA after incubation for 30 days. Conidia are ampule shaped, one sepate, constricted at the septum, hyaline, and guttulate. However, not much difference was obtained in colony diameter and size of conidia. Seven isolates of the rDNA of ITS region was amplified by ITS4/ITS6 primers and ranged from 565~615 bp in the isolates. On BLAST, isolates showed high homologies (96~100%) with Marssonina coronaria. Phylogentic tree depicted that the isolates were closely clustered with Chinese isolates with accession numbers FJ606800, EU329733, EU329735, FJ618562, FJ618561 and FJ618560. The present study demonstrated the genetic relationships among the isolates of Marssonina coronaria and indicated that ITS rDNA sequence data were potentially useful in taxonomic species determination.


Keywords: Marssonina coronaria, Morphological, Pathogenicity, ITS regions
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How to cite this article:

Phurailatpam, S., J.N. Sharma, A. Brakta, Sushma Sharma and Shweta Sharma. 2019. Morphological and Molecular Characterization of Marssonina coronaria (Ellis & J.J. Davis) J.J. Davis Causing Premature Leaf Fall.Int.J.Curr.Microbiol.App.Sci. 8(6): 2762-2769. doi: https://doi.org/10.20546/ijcmas.2019.806.333