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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 2679-2688
DOI: https://doi.org/10.20546/ijcmas.2019.806.322


Ultrastructural Studies to Establish the Mode of Action of Pseudomonas fluorescens against Aspergillus flavus using Scanning Electron Microscope
M. Ravi Teja1*, K. Vijay Krishna Kumar2 and H. Sudini3
1College of Agriculture, Professor Jayashankar Telangana State Agricultural University (PJTSAU), Rajendranagar, Hyderabad-500030, Telanagana, India
2Acharya N.G. Ranga Agricultural University, Guntur, Andhra Pradesh- 522034, India
3International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Telangana-502324, India
*Corresponding author
Abstract:

In Groundnut aflatoxin contamination is a major problem which affects the quality of the seed. These aflatoxins have carcinogenic, hepatotoxic, teratogenic and immuno-suppressive effects which were produced by Aspergillus flavus and A. parasiticus. For the management of aflatoxin problem in groundnut, use of plant growth-promoting rhizobacteria (PGPR) is a viable and sustainable option. Our present study is aimed at determining the mode of action of these PGPR (Pseudomonas fluorescens) using Scanning Electron Microscope (SEM). A superior P. fluorescens isolate (Pf7) identified from our studies in dual culture studies and in vitro seed colonization assay (IVSC) was screened against toxigenic isolate of A. flavus (AFT5B). In SEM, Pf7 exhibited both antibiosis and hyperparasitism. In antibiosis, deformation and coiling of hyphae of test fungus was noticed. Further, the mycelium appeared thread like, wrinkled and flaccid. Conidiophores are twisted near the zone of inhibition. In hyperparasitism, a white slimy growth of bacterial cells was seen on mycelia of test fungus. Further, structural disintegration of conidiophores and conidia of A. flavus was noticed. Overall, our results suggested the efficacy of Pf7 in reducing A. flavus infection and aflatoxin contamination through hyperparasitism and antibiosis.


Keywords: Groundnut, Aflatoxins, Aspergillus flavus, Pseudomonas fluorescens, SEM, Antibiosis, Hyperparasitism
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How to cite this article:

Ravi Teja, M., K. Vijay Krishna Kumar and Sudini, H. 2019. Ultrastructural Studies to Establish the Mode of Action of Pseudomonas fluorescens against Aspergillus flavus using Scanning Electron Microscope.Int.J.Curr.Microbiol.App.Sci. 8(6): 2679-2688. doi: https://doi.org/10.20546/ijcmas.2019.806.322