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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 2670-2678
DOI: https://doi.org/10.20546/ijcmas.2019.806.321


Extracting gDNA Suitable for Molecular Applications from Sugarcane, and its Phytopathogens
Sangeeta Srivastava* and Prashant Shekhar Gupta
Crop Improvement Division, ICAR-Indian Institute of Sugarcane Research, Lucknow-226002, India
*Corresponding author
Abstract:

DNA fingerprinting, estimation of molecular genetic diversity, marker assisted breeding and molecular diagnostic tools are the need of the hour for sugarcane crop improvement. Efficient extraction of genomic DNA (gDNA) free from polysaccharides, polyphenols, RNA and other major contaminants is a key to all these molecular activities. We present herewith, a modified DNA extraction protocol that has been optimized for isolating gDNA from endergonic and hardy sugarcane plant and its phytopathogens and endophytic fungi. The protocol has worked equally well in a number of monocot and dicot plants including rice, maize, linseed, Sesbania, opium poppy, Hyoscyamus, phytoplasma infected sugarcane leaves and smut infected sugarcane whip. The success of this modified protocol was assessed by polymerase chain reaction (PCR)-based amplification and sequencing of amplicons, universality and reliability.


Keywords: gDNA extraction, Sugarcane, Phytoplasma, C. falcatum, rRNA
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How to cite this article:

Sangeeta Srivastava and Prashant Shekhar Gupta. 2019. Extracting gDNA Suitable for Molecular Applications from Sugarcane, and its Phytopathogens.Int.J.Curr.Microbiol.App.Sci. 8(6): 2670-2678. doi: https://doi.org/10.20546/ijcmas.2019.806.321