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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 2456-2464
DOI: https://doi.org/10.20546/ijcmas.2019.806.293


Molecular Divergence Studies through RAPD Markers in Potato (Solanum tuberosum L.) Germplasm
Anamika Verma1* and Dhirendra Singh2
1Department of Horticulture, Lovely Professional University, Phagwara, Punjab, India
2Department of Vegetable Science, College of Agriculture, G B Pant University of Agriculture & Technology, Pantnagar, U.K. 263145, India
*Corresponding author
Abstract:

Genetic diversity using Random Amplified Polymorphic DNA (RAPD) markers was assessed in forty eight Indian potato germplasm. Twenty RAPD markers amplified a total of ninety-one different loci that exhibited 82.30 per cent polymorphism among 48 potato germplasm. The PIC value ranged from 0.27 to 0.55. All the loci amplified by the primer which were found to be polymorphic varied in size from <50bp to >1500bp. The Jaccard’s similarity coefficient was found to vary from 0.22 to 0.87. The maximum genetic similarity (0.87) was found between Kufri Badshah and Kufri Khyati and lowest (0.22) between Pant Sel.-01-15 and Pant Sel.-09-57. UPGMA based dendrogram ordered the populations of genotypes into nine clusters. The most diverse groups found were Cluster II and cluster IX followed by cluster I and VII with Cluster II. RAPD markers were found to be sufficiently sensitive to detect genetic variation among various germplasm, which will be useful for selecting genetically distinct germplasm in potato breeding programme.


Keywords: Genetic diversity, potato, RAPD, Jaccard’s Coefficient of similarity, UPGMA, PCR amplification, DNA isolation
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How to cite this article:

Anamika Verma and Dhirendra Singh. 2019. Molecular Divergence Studies through RAPD Markers in Potato (Solanum tuberosum L.) Germplasm.Int.J.Curr.Microbiol.App.Sci. 8(6): 2456-2464. doi: https://doi.org/10.20546/ijcmas.2019.806.293