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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 2391-2398
DOI: https://doi.org/10.20546/ijcmas.2019.806.284


Studies on in vitro Culture of Coconut var. Chowghat Orange Dwarf through Direct Organogenesis
D.S. Sushmitha, R. Renuka* and D. Chandrakala
Plant Tissue Culture Laboratory, Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore – 641003, Tamil Nadu, India
*Corresponding author
Abstract:

Coconut has a wide range of applications from culinary to industrial areas. Loss of coconut trees due to the devastating effect of deadly diseases and the natural aging of the plants demand rejuvenation of orchards with elite seedlings. A conventional breeding program is not sufficient to meet the growing need for healthy planting materials. However, in vitro culture studies can pave the way for mass multiplication. In this study, the effect of TDZ on sliced mature embryo explants through direct organogenesis of coconut var. Chowghat Orange Dwarf (COD) was investigated. The treatment Y3 + 150µM TDZ recorded highest shoot induction (90.91 %) and shoot regeneration (72.73 %). A higher concentration of TDZ reduced the frequency of shoot organogenesis. Well-grown shoots were transferred to rooting media and plantlets were subjected to primary hardening.


Keywords: Coconut, Direct organogenesis, TDZ, Sliced mature embryo, Shoot regeneration, Chowghat Oragne Dwarf
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How to cite this article:

Sushmitha, D.S., R. Renuka and Chandrakala, D. 2019. Studies on in vitro Culture of Coconut var. Chowghat Orange Dwarf through Direct Organogenesis.Int.J.Curr.Microbiol.App.Sci. 8(6): 2391-2398. doi: https://doi.org/10.20546/ijcmas.2019.806.284