Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(1): 579-591
DOI: http://dx.doi.org/10.20546/ijcmas.2017.601.071


Ophiocordyceps pulvinata Induces Caspase-Dependent Apoptosis through Mitochondrial Impairment in Human Leukemia Cells

Yukiko Ogawa1*, Osamu Morinaga2, Nobuo Yahagi3, Remiko Yahagi3 and Hidemitsu Kobayashi4

1Divisions of Infection Control and Prevention, Department of Pharmacy, Faculty of Pharmaceutical Science, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan
2Department of Natural Medicines, Daiichi University of Pharmacy, 22-1 Tamagawa-cho, Minami-ku, Fukuoka, 815-8511, Japan
3Natural Medical Plant and Microbiological Organism Research, Mamurogawa-machi, Mogami-gun, Yamagata, 999-5604, Japan
4Divisions of Microbiology, Department of Pharmacy, Faculty of Pharmaceutical Science, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan
*Corresponding author
Abstract:

Cordyceps is a genus of fungi from the Cordycipitaceae family that grows using host insects as the nutritional source. This genus comprises approximately 500 species worldwide. In the present study, culturing 16 species from 3 genuses of Chu-Soh, namely, Cordyceps, Ophiocordyceps, and Isaria, were successfully artificially cultured We then specifically investigated the antitumor effects of Ophiocordyceps pulvinata on human cancer cells. Secondary metabolites of O. pulvinata (OP-FD) showed a significant anti-proliferative effect on breast cancer and leukemia cells at low concentrations but not on gastric and pancreatic cancer cells at the same concentrations. Virtually no effect was observed in normal human dermal fibroblasts (NHDF) cells. As OP-FD did not lose antitumor activity on heating, further fractionation with organic solvents was conducted to investigate the antitumor effects. The hexane (OP-He) and chloroform (OP-CH) fractions showed a potent cytotoxic effect on human breast cancer and leukemia cells at lower concentrations than OP-FD. Furthermore, OP-He-induced cell death was characterized by mitochondrial impairment, loss of mitochondrial membrane potential (ΔΨm), caspase-9 and-3 activation, sub-G1 phase DNA, chromatin condensation, and DNA fragmentation. These results indicate that the cytotoxicity of O. pulvinata to leukemia cells is due to apoptosis via the caspase-dependent mitochondrial pathway.


Keywords: Ophiocordyceps pulvinata, antitumor activity, apoptosis, medicinal plants, leukemia cells.
Download this article as Download

How to cite this article:

Yukiko Ogawa, Osamu Morinaga, Nobuo Yahagi, Remiko Yahagi and Hidemitsu Kobayashi. 2017. Ophiocordyceps pulvinata Induces Caspase-Dependent Apoptosis through Mitochondrial Impairment in Human Leukemia Cells.Int.J.Curr.Microbiol.App.Sci. 6(1): 579-591. doi: http://dx.doi.org/10.20546/ijcmas.2017.601.071