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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 2054-2058
DOI: https://doi.org/10.20546/ijcmas.2019.806.245


Evaluation of a Multiplex PCR Assay for Rapid Diagnosis of Fowl Typhoid
Susmita Pal, Samir Dey*, Kunal Batabyal, Abhiroop Banerjee, Siddhartha Narayan Joardar and Devi Prasad Isore
Department of Veterinary Microbiology, West Bengal University of Animal and Fishery Sciences (WBUAFS), Belgachia, Kolkata-700037, India
*Corresponding author
Abstract:

Despite Fowl typhoid (FT) eradication in commercial poultry of some parts of the world, outbreak threats are not eliminated in poultry of developing countries. In the current study, we reported molecular identification of Salmonella gallinarum in Indian isolates from backyard poultry by a multiplex PCR assay. Boiled culture lysates of Salmonella Gallinarum isolates (n=2) from an outbreak showed positive amplifications of glgC and speC genes in duplex PCR with 174 bp and 252 bp products, respectively. In multiplex PCR assay, primers for salmonella plasmid virulence gene (spvC) were also added in same reaction mixture. No amplifications were noticed in negative controls with other Salmonella serotypes and non –Salmonella organisms. This multiplex PCR assay was found to be rapid and specific in diagnosis of fowl typhoid.


Keywords: Fowl typhoid, glgC, Multiplex, Poultry, PCR, Salmonella gallinarum, speC
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How to cite this article:

Susmita Pal, Samir Dey, Kunal Batabyal, Abhiroop Banerjee, Siddhartha Narayan Joardar and Devi Prasad Isore. 2019. Evaluation of a Multiplex PCR Assay for Rapid Diagnosis of Fowl Typhoid.Int.J.Curr.Microbiol.App.Sci. 8(6): 2054-2058. doi: https://doi.org/10.20546/ijcmas.2019.806.245