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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(1): 563-568
DOI: http://dx.doi.org/10.20546/ijcmas.2017.601.069


A Leptospirosis Workup by Evaluation of Efficiency of Two Rapid Immunodiagnostic Tests in Comparison with Microscopic Agglutination Test
H.R. Ganesh, Vimal Kumar Karnaker* and Veena A. Shetty
Department of Microbiology, K.S.Hegde Medical Academy, Deralakatte, Nithyanandanagar Post, Mangalore-575018, India
*Corresponding author
Abstract:

Serological tests plays an important role in the diagnosis of leptospirosis as many clinical manifestations simulate diseases like dengue fever, hepatitis, Enteric fever and Malaria. Laborious and time consuming MAT, a gold standard test led to the introduction of Immunochromatography based tests, card tests and various ELISA kits for rapid diagnosis of acute leptospirosis by peripheral laboratories. The objective of our study was to know the efficacy of the tests like Leptocheck WB IgM(ICT), PanBio IgM ELISA(ELISA), with MAT. Hundred and eleven serum samples from 54 patients with clinical suspicion of leptospirosis and 50 serum from asymptomatic subjects was tested for IgM antibodies by the kits. Out of 54 serum collected, 42.59% were positive by ICT, 48.14% by ELISA, and 53.7% by MAT. ICT on comparison with MAT showed sensitivity of 75 % and 77.4%, Specificity of 96% and 95.6%, Positive Predictive value (PPV) 95.7% and 96%, Negative Predictive Value (NPV) 77.4% and 75.9% respectively and Kappa value 0.707.ELISA recorded a sensitivity of 89.7% and 90.3%, specificity of100%, PPV of 100%, NPV 89.3% and 88.46 respectively and Kappa value 0.889 with MAT. As ELISA was more sensitive and specific in picking up leptospiral cases early in the infection when compared to ICTs,thusshould be included with ICTs in laboratories to increase the detection of suspected cases of acute leptospirosis with history of 4-6 days of febrile illness.


Keywords: Immunochromatography test, ELISA, Leptospirosis, Microscopic agglutination tests.
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How to cite this article:

Ganesh, H.R., Vimal Kumar Karnaker, Veena A. Shetty. 2017. A Leptospirosis Workup by Evaluation of Efficiency of Two Rapid Immunodiagnostic Tests in Comparison with Microscopic Agglutination Test.Int.J.Curr.Microbiol.App.Sci. 6(1): 563-568. doi: http://dx.doi.org/10.20546/ijcmas.2017.601.069