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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 1996-2007
DOI: https://doi.org/10.20546/ijcmas.2019.806.238


Isolation, Identification, Molecular Characterization and Antibiogram of E. coli Isolates from Neonatal Calves
Shailendri Gupta1*, Abhishek1, Sameer Shrivastava2 and Asha K. Verma3
1Division of Bacteriology & Mycology, ICAR-IVRI, Izatnagar-243122, India
2Division of Veterinary Biotechnology, ICAR-IVRI, Izatnagar-243122, India
3Division of Veterinary Public Health, ICAR-IVRI, Izatnagar-243122, India
*Corresponding author
Abstract:

Neonatal calf diarrhoea (NCD) is a major threat to dairy industries. It is a multi-etiology disease with E. coli as the predominant cause. Emergence of antimicrobial resistance is of utmost concern because it has rendered the present day antibiotics less effective. The present study aims for isolation, molecular characterization and antimicrobial resistant profiling of E. coli from neonatal calves. One hundred two fecal samples were collected from neonatal calves for isolation of E. coli. All the isolates showed positive result on biochemical analysis. But only 78.43% (80/102) of them were found positive for uspA (universal stress protein) gene. The nucleotide sequences flanking the gene encoding the uspA and was found to be highly specific for E. coli. The antibiogram of E. coli isolates by Kirby Bauer disc diffusion method revealed highest resistance against kanamycin (97%) and lowest against gentamicin (5.86%). Eighty three percent (85/102) isolates were MDR. 39 (38.24%) isolates were phenotypic ESBL producers (by combination disc diffusion method) and 22 (21.57%) isolates were phenotypic carbapenem resistant (against Ertapenem, Meropenem, Imipenem, Doripenam) Hence, surveillance based study and search for an alternative to antibiotics is needed to address the global issue of the antimicrobial resistance.


Keywords: Neonatal calf diarrhoea (NCD), multidrug resistant MDR E. coli, uspA gene, Antibiogram, Carbapenem Resistant, ESBL Producers
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How to cite this article:

Shailendri Gupta, Abhishek, Sameer Shrivastava and Asha K. Verma. 2019. Isolation, Identification, Molecular Characterization and Antibiogram of E. coli Isolates from Neonatal Calves.Int.J.Curr.Microbiol.App.Sci. 8(6): 1996-2007. doi: https://doi.org/10.20546/ijcmas.2019.806.238