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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 1900-1908
DOI: https://doi.org/10.20546/ijcmas.2019.806.228


Morphological and Molecular Characterization of Magnaporthe grisea and Bio-Efficacy of Bacillus Strains against M. grisea
Swastisarita Shaw1*, S.R. Prabhukarthikeyan1, U. Keerthana1, S. Aravindan1, Manoj Kumar Yadav1, S. Raghu1, Mathew S. Baite1, R. Naveenkumar1, Sabyasachy Parida2, Gayatree Panda2 and P.C. Rath1
1ICAR-National Rice Research Institute, Cuttack, Odisha, India
2MPC Autonomous College, Takatpur, Baripada, Odisha, India
*Corresponding author
Abstract:

A study was undertaken to isolate, identify and characterize the rice blast pathogen M. grisea from different rice growing regions. In the present study, ten isolates of M. grisea were categorized into different groups based on colony colour and texture. PCR was performed to identify the M. grisea isolates using the universal primers of 18S (ITS 1) and 28S (ITS 4). The PCR reaction allowed amplifying the fungal ITS fragments of 550 bp. All the isolates had the expected specific size of 550 bp which depicts molecular based confirmation of M. grisea. Biocontrol approaches help to develop an eco-friendly sustainable management strategy for controlling the diseases. In this connection, totally ten strains of Bacillus spp. were screened against six isolates of M. griseato test their efficacy. Among the strains, BS5 showed maximum mycelial growth inhibition followed by BS26.


Keywords: Morphological, Molecular, Magnaporthe grisea, Bacillus
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How to cite this article:

Swastisarita Shaw, S.R. Prabhukarthikeyan, U. Keerthana, S. Aravindan, Manoj Kumar Yadav, S. Raghu, Mathew S. Baite, R. Naveenkumar, Sabyasachy Parida, Gayatree Panda and Rath, P.C. 2019. Morphological and Molecular Characterization of Magnaporthe grisea and Bio-Efficacy of Bacillus Strains against M. grisea.Int.J.Curr.Microbiol.App.Sci. 8(6): 1900-1908. doi: https://doi.org/10.20546/ijcmas.2019.806.228