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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 1765-1772
DOI: https://doi.org/10.20546/ijcmas.2019.806.211


Identification of Trichoderma Isolates from Tobacco Growing Regions of West Godavari District, Based on Sequence Analysis of ITS Region of rDNA and Morphological Variations in the Strains
Santosh Swamy1,3*, U. Sreedhar2,3, R.D. Prasad4, B.S.R. Reddy1 and P. Sudhakar3
1Research Department, Agri Business Division, ITC Limited, Rajahmundry 533 103, A.P, India 2Central Tobacco Research Institute, Rajahmundry, A.P, India 3Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, A.P., India 4Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad, Telangana, India
*Corresponding author
Abstract:

The aim of this study was to determine the phylogenetic relationships of Trichoderma isolates obtained from NLS Tobacco region. Utilizing the sequence analysis of internal transcribed spacer-1 (ITS-1) region of the ribosomal DNA the rDNA of Five Trichoderma isolates was amplified by polymerase chain reaction (PCR) using universal primers (ITS-1 and ITS-4). PCR products were purified and these purified products were used to amplify the ITS-4 region of the five Trichoderma isolates. The amplified DNA was sequenced and aligned against ex-type strain sequences from TrichoBLAST and established Trichoderma taxonomy. Two isolates were positively identified as Trichoderma harzianum and three isolates were identified as Trichoderma aesperulum which were used as an outgroup in these analyses. The ITS-1 region sequences have been used as the reference’s sequence and this could be further used for future study involving the identification and taxonomy of Trichoderma Amplification of ITS-1 region of the rDNA. This method proved as a rapid and reliable technique for identifying Trichoderma strains successfully.


Keywords: Trichoderma, Amplification, Distribution
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How to cite this article:

Santosh Swamy, U. Sreedhar, R.D. Prasad, B.S.R. Reddy and Sudhakar, P. 2019. Identification of Trichoderma Isolates from Tobacco Growing Regions of West Godavari District, Based on Sequence Analysis of ITS Region of rDNA and Morphological Variations in the Strains.Int.J.Curr.Microbiol.App.Sci. 8(6): 1765-1772. doi: https://doi.org/10.20546/ijcmas.2019.806.211