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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 1676-1683
DOI: https://doi.org/10.20546/ijcmas.2019.806.200


Morphological and Ultrastructure of Developmental Stages of Somatic Embryos of Popular Banana Cultivars
Ruma Debbarma1, D. Sudhakar2, K.K. Kumar2 and K. Soorianathasundaram1*
1Department of Fruit Science, HC & RI, TNAU, Coimbatore-03, Tamilnadu, India
2Department of Plant Biotechnology, CPMB & B, TNAU, Coimbatore-03, Tamilnadu, India
*Corresponding author
Abstract:

Somatic embryogenesis based plant regeneration is a pre-requisite for genetic transformation of banana. Mass multiplication of banana through somatic embryogenesis is also viewed as an economically viable alternative to commonly used shoot tip based method. Banana somatic embryogenesis response was studied in three popular banana cultivars, Grand Naine, Rasthali and Hill banana ‘Virupakshi’. Immature male flower bud explant was used for embryogenic callus induction. Different morphological types of calli were produced during callus induction that includes both embryogenic and non-embryogenic calli. White translucent pro-embryogenic calli were found to be suitable for establishment of embryogenic cell suspension (ECS). Regeneration potential of ECS was assessed and the development of different stages of embryo viz., globular, scutellum, and coleoptillar stages was observed under scanning electron microscope to understand their ultra-structure. The differences in the duration of developmental stages and regeneration potential could be attributed to the genomic differences of cultivars involved.


Keywords: Somatic embryogenesis, embryogenic calli, non-embryogenic calli, cell suspension cultures, somatic embryo, scanning electron microscope
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How to cite this article:

Ruma Debbarma, D. Sudhakar, K.K. Kumar and Soorianathasundaram, K. 2019. Morphological and Ultrastructure of Developmental Stages of Somatic Embryos of Popular Banana Cultivars.Int.J.Curr.Microbiol.App.Sci. 8(6): 1676-1683. doi: https://doi.org/10.20546/ijcmas.2019.806.200