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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 1496-1506
DOI: https://doi.org/10.20546/ijcmas.2019.806.180


Phenotypic and Molecular Characterization of Chaetomium globosum (Gustav Kunze) from Different Microhabitats of Tamil Nadu
M.V. Ruppavalli1, M. Muthamilan1*, S. Nakkeeran1 and K.S. Subramanian2
1Department Plant Pathology, Tamil Nadu Agricultural University, Coimbatore – 641003, Tamil Nadu, India
2Department of Nano Science and Technology, Tamil Nadu Agricultural University, Coimbatore – 641003, Tamil Nadu, India
*Corresponding author
Abstract:

The biocontrol agent Chaetomium globosum is widely prevalent in soil and other cellulose containing substrates. Fifteen isolates of C. globosum was isolated from different microhabitats of Tamil Nadu using baiting, direct isolation from decomposed materials and through serial dilution technique. All the fifteen isolates were phenotypically identified by their colony growth and perithecia. The colour of the colony varied from greenish white to yellowish white with radial and fluffy mycelial growth pattern. The pycnidia of different Chaetomium isolates varied in their shapes like ovoid, lemoniform, elliptical or slightly elliptical with an apical germ pore. The size of ascomata varied from 105.1 – 593.45 x 51.21 - 387.41 µm dia. Comparison of perithecial production between the C.globosum isolates indicated that the number of perithecia was more in TNAU-Cg 101 and TNAU-Cg 105 isolates. Ascospores were brown in colour and size varied from 8 - 14 µm dia. The sporulation was higher in TNAU-Cg101, TNAU-Cg 102 and TNAU-Cg105 isolates. Besides, all fifteen isolates were confirmed as C.globosum, by amplification through internal transcribed spacer rDNA sequences bearing the accession numbers MK587669, MK590290, MK592857, MK603862, MK592858, MK828135, MK821416, MK823129, MK828197, MK603941, MK828136, MK757844, MK820066, MK828133 and MK828134. Phylogenetic analysis of the fifteen C. globosum isolates, indicated that all the isolates were clustered into a single group.


Keywords: Chaetomium globosum, ascomata, Decomposed material, Baiting technique
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How to cite this article:

Ruppavalli, M.V., M. Muthamilan, S. Nakkeeran and Subramanian, K.S. 2019. Phenotypic and Molecular Characterization of Chaetomium globosum (Gustav Kunze) from Different Microhabitats of Tamil Nadu.Int.J.Curr.Microbiol.App.Sci. 8(6): 1496-1506. doi: https://doi.org/10.20546/ijcmas.2019.806.180