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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 1003-1010
DOI: https://doi.org/10.20546/ijcmas.2019.806.122


Isolation and Identification of Non-Fermentative Gram Negative Bacilli
B. Ramchandra Reddy1, K. Shirisha2*, M. Singh and V. Kabra 
1Department of Microbiology, S.V.S. Medical College, Mahabubnagar, Telangana, India
2Department of Microbiology, Malla Reddy Medical College for women, Hyderabad, Telangana, India
*Corresponding author
Abstract:

Nonfermentative group of bacteria have been incriminated as emerging opportunistic pathogens, especially from hospital settings due to their increasing frequency of isolation from clinical specimens. Nonfermenters show natural resistance to a wide range of commonly used antibiotics. This study includes 50 isolates of nonfermenters from different clinical specimens like Pus ,Sputum, blood and urine obtained from patients attending SVS Medical college & Hospital from Jan2007 to March 2008.Out of 50 nonfermenters, Pseudomonas sp.(82%), Acinetobacter sp.(14%), Alcaligens sp.(4%) were isolated. Sensitivity pattern was Piperacillin (60%), Amikacin (52%), Ciprofloxacin (46%), Ceftazidime (46%), Cefotaxime (44%), Cefi-pime (36%), Gentamycin (24%). This study showed that non-fermenters were frequently isolated from clinical specimens and that they are becoming resistant to the routinely used antibiotics and hence should be taken seriously rather than regarding them as contaminants or commensals.


Keywords: Gram Negative Bacilli, Non-Fermentative, Identification, Isolation
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How to cite this article:

Ramchandra Reddy, B.K. Shirisha, M. Singh and Kabra, V. 2019. Isolation and Identification of Non-Fermentative Gram Negative Bacilli.Int.J.Curr.Microbiol.App.Sci. 8(6): 1003-1010. doi: https://doi.org/10.20546/ijcmas.2019.806.122