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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(1): 423-432
DOI: http://dx.doi.org/10.20546/ijcmas.2017.601.051


Study of Antibacterial Efficacy of Methanolic and Aqueous Leaf Extracts of Scoparia dulcis on Some Human Pathogenic Bacteria
Sophy Jose* and M.P. Sinha
Department of Zoology, Ranchi University, Ranchi-834008, Jharkhand, India
*Corresponding author
Abstract:

Scoparia dulcis is a well known traditional medicinal plant used in various indigenous systems of medicine. It is widely distributed throughout India. The present study provides phytochemical and antimicrobial details of the methanolic and aqueous leaf extracts of Scoparia dulcis against clinically important human pathogens viz. Staphylococcus aureus, Proteus mirabilis, Salmonella typhi, Vibrio cholerae and Bacillus subtilis. The phytochemical analysis carried out revealed the presence of flavanoids, glycosides, alkaloids, tannins, steroids and many other metabolites and the absence saponins. Minimum inhibitory concentration (MIC) assay was determined for the extract. The methanolic and aqueous extract showed toxicity against all the bacteria, V. Chlolerae and P.mirabilis being highly susceptible with a zone of inhibition of 4 mm at 10mg/ml and 2 mm  at 10mg/ml respectively in agar diffusion method. The broth dilution method showed more pronounced antimicrobial activity through 100% inhibition for all the pathogens in the range of 1-32mg/mL concentration. The MIC for S.typhi and B.subtilis in methanolic solution was 32mg/mL, for P.mirabilis 16mg/mL, for S.aureus  8mg/mL  and V.cholerae 1mg/mL. The MIC for S.aureus and V.cholerae in aqueous solution was 16mg/mL, for P.mirabilis 4mg/mL, S. typhi 8mg/mL and for B.subitlis 32mg/mL.   


Keywords: Scoparia dulcis, Pathogens, Antibacterial activity,Extracts.
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How to cite this article:

Sophy Jose and M.P. Sinha. 2017. Study of Antibacterial Efficacy of Methanolic and Aqueous Leaf Extracts of Scoparia dulcis on Some Human Pathogenic Bacteria.Int.J.Curr.Microbiol.App.Sci. 6(1): 423-432. doi: http://dx.doi.org/10.20546/ijcmas.2017.601.051