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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 443-455
DOI: https://doi.org/10.20546/ijcmas.2019.806.050


Isolation and Characterization of Indole Acetic Acid (IAA) Producing Tomato Rhizobacterium Pseudomonas sp VSMKU4050 and its Potential for Plant Growth Promotion
R. Kalimuthu1,2, P. Suresh2, G. Varatharaju2, N. Balasubramanian3,  K.M. Rajasekaran1* and V. Shanmugaiah2*
1Department of Botany, Madura College, Madurai – 625 011, Tamil Nadu, India
2Department of Microbial Technology, 3Department of Immunology, School of Biological Sciences, Madurai Kamaraj University, Madurai - 625 021, Tamil Nadu, India
*Corresponding author
Abstract:

Indian soils are rich in microbial diversity, especially fluorescent pseudomonads (FPs) have drawn much attention worldwide because of their plant growth promotion ability by the production of plant growth promoting substance like Indole acetic acid (IAA). In this context, the present study explored for optimization and characterization of IAA production by our isolate Pseudomonas sp VSMKU 4050. The maximum IAA production was observed in King’s B broth (KBB) supplemented with 0.7% L – tryptophan. The KBB medium was recognized as the best medium for IAA production, while the maximum IAA production was recorded at 35° C and pH 7.0 for the production of 6.80 µg/ml and 11.50 µg/ml respectively. The specific spot was found from the ethyl acetate extract, IAA has similarity to authentic IAA with the same Rf value of 0.87. Further IAA production was confirmed in our isolate VSMK4050 by UV and IR spectral studies. The selected strain VSMK4050 was treated with tomato seeds (4 X 10 8), significantly enhance the growth of tomato seedlings in non sterile and sterile soil compared to other treatments (16.5cm, 5.2cm and 18.2cm, 8cm). Similarly cells free culture filtrate significantly enhances the tomato seedlings in both non sterile and sterile soil compared to other treatments. Based on the results we suggested that our isolate VSMKU4050 could be used as a significant inoculum for the enhancement of tomato seedlings.


Keywords: Indole acetic acid, Pseudomonas, Plant growth promotion, TLC and L - tryptophan
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How to cite this article:

Kalimuthu, R., P. Suresh, G. Varatharaju, N. Balasubramanian, K.M. Rajasekaran and Shanmugaiah, V. 2019. Isolation and Characterization of Indole Acetic Acid (IAA) Producing Tomato Rhizobacterium Pseudomonas sp VSMKU4050 and its Potential for Plant Growth Promotion.Int.J.Curr.Microbiol.App.Sci. 8(6): 443-455. doi: https://doi.org/10.20546/ijcmas.2019.806.050