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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 379-389
DOI: https://doi.org/10.20546/ijcmas.2019.806.043


Evaluation of In vitro Antioxidant Activity of Nelumbo nucifera Leaf Extract and its Potential Application as Antibacterial Agent against Fish Pathogens
Mudasir Maqsood Hakim1*, Nazir Ahmad Ganai2, Syed Mudasir Ahmad1, Oyas Ahmad Asimi3, Tariq Raja2, Feroz Ahmad Shah4, Jalal-ul-Din Parrah5 and Riaz Ahmad Shah1
1Division of Animal Biotechnology, Faculty of Veterinary Sciences & Animal Husbandry, SKUAST-Kashmir, India
2Division of Animal Genetics and Breeding, Faculty of Veterinary Sciences & Animal Husbandry, SKUAST-Kashmir, India
3Division of Fish Nutrition and Biochemistry, Faculty of Fisheries, SKUAST-Kashmir, India
4Division of Aquatic Animal Health & Management, Faculty of Fisheries,
SKUAST-Kashmir, India
5Mountain Livestock Research Institute, SKAUST-Kashmir, India 
*Corresponding author
Abstract:

The aim of this study was to determine the antioxidant and antibacterial property of Nelumbo nucifers (lotus) leaf extract. Total phenolic content and DPPH (2,2-diphenyl-1-picrylhydrozyl) scavenging methods were used to evaluate the antioxidant property of crude extract. DPPH scavenging capacity of extract varied significantly (p<0.05) depending on the concentration, except 5.5mg/ml and 7 mg/ml concentrations. The maximum concentration (10mg/ml) of the extract showed the highest scavenging effect (57.75%), whereas the lowest concentration (0.5mg/ml) of the extract showed the least scavenging capacity (9.30 %). The phenolic contents exhibited a similar trend to that of DPPH. Total phenolic compounds increased with the increasing concentration of Nelumbo nucifera leaf extract. Disc diffusion and broth micro-dilution methods showed bactericidal property of lotus leaf extract. Water, acetone-water and ethanol-water based extracts were tested against selected gram-positive (Staphylococcus aureus) and gram-negative (Aeromonas hydrophila, Pseudomonas fluorescens) fish bacterial pathogens. The broth micro-dilution method with TTC (2,3,5-triphenyl tetrazolium chloride) to indicate the viability of aerobic bacteria was found to be the best alternative method.


Keywords: Louts leaf extract, Antibacterial, Antioxidant, Aeromonas hydrophila, Fish Pathogen, Aquaculture, Fish Nutrition
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How to cite this article:

Mudasir Maqsood Hakim, Nazir Ahmad Ganai, Syed Mudasir Ahmad, Oyas Ahmad Asimi, Tariq Raja, Feroz Ahmad Shah, Jalal-ul-Din Parrah and Riaz Ahmad Shah. 2019. Evaluation of In vitro Antioxidant Activity of Nelumbo nucifera Leaf Extract and its Potential Application as Antibacterial Agent against Fish Pathogens.Int.J.Curr.Microbiol.App.Sci. 8(6): 379-389. doi: https://doi.org/10.20546/ijcmas.2019.806.043