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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(6): 317-329
DOI: https://doi.org/10.20546/ijcmas.2019.806.036


Molecular Characterization of CTX-M15 Beta Lactamase Producing Escherichia coli Isolates in Intensive Care Units
Rehab M. Ateya1*, Ghada E. Amr1, Hanan E. Mohamed1 and Neveen M. Gamil2
1Departments of Clinical Pathology, Faculty of Medicine, Zagazig University, Egypt
2Anaesthesia and Intensive Care, Faculty of Medicine, Zagazig University, Egypt
*Corresponding author
Abstract:

The rapid emergence of multiresistant microbial pathogens is a serious threat to human health. Extended spectrum beta lactamase (ESBL)-producing Escherichia coli is a superbug causing worldwide outbreaks, CTX-M has become the most commonly detected ESBL genotype; these enzymes have become predominant worldwide, the most common type of CTX-M reported in various geographic regions is CTX-M15. A total of 80 Escherichia coli (E. coli) isolates were recovered from different clinical samples of intensive care units (ICUs) patients. These isolates were identified by Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS), in vitro antimicrobial susceptibility was investigated by VITEK 2 instrument, ESBL production was assessed phenotypically and ESBL genes (TEM, SHV and CTX-M15) confirmed genotypically by PCR and further confirmation for CTX-M15 by DNA sequencing. More than 50% of ESBL E. coli isolates were multidrug resistant; while carbapenams remains the most active compound among the studied isolates. Among ESBL E. coli, CTX-M-15 was 90% which was confirmed by sequencing. To our knowledge, this is the first report to study CTX-M-15 in E. coli isolates in Sharkia governorate. This study showed a high rate of ESBL E. coli with the widespread dissemination of CTX-M-15 which emphasize the need for employing an excellent management program in antibiotic therapy.


Keywords: CTX-M15, ICUs, E. coli, PCR, ESBL, Sequencing
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How to cite this article:

Rehab M. Ateya, Ghada E. Amr, Hanan E. Mohamed and Neveen M. Gamil. 2019. Molecular Characterization of CTX-M15 Beta Lactamase Producing Escherichia coli Isolates in Intensive Care Units.Int.J.Curr.Microbiol.App.Sci. 8(6): 317-329. doi: https://doi.org/10.20546/ijcmas.2019.806.036