Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(1): 396-410
DOI: http://dx.doi.org/10.20546/ijcmas.2017.601.048


Anti-Virulence Activity of Selected Natural Products against Pseudomonas aeruginosa
Ahmed Ahmed Abd El-Aziz, Tarek El-Said El-Banna, Fatma Ibrahim Sonbol and Shymaa Mohamed Sallam*
Pharmaceutical Microbiology Department, Faculty of Pharmacy, Tanta University, Egypt
*Corresponding author
Abstract:

Pseudomonas aeruginosa is considered as the epitome of an opportunistic pathogens of hospitalized patients. A total of 159 clinical specimens were recovered and molecular identified by PCR assay. The number of isolates which identified as P. aeruginosa was 101 clinical isolates. Considering the effect of 14 natural products/extracts on some virulence factors of MDR isolates,  sub inhibitory concentration (½ MIC) of Syzygium aromaticum (clove) buds extract inhibit the activity of alkaline protease enzyme on skimmed milk agar. The swarming motility was decreased while phospholipase C enzyme activity was increased by sub inhibitory conc. of clove buds extract. Biofilm formation of P. aeruginosa was inhibited by Psidium guajava (guava) leaves extract at ½ MIC (showed pink colonies on congo red agar).The effect of different conc. of Psidium guajava leaves extract on outer membrane proteins of Pseudomonas aeruginosa isolate was determined by SDS-PAGE  and analyzed using Image J program. Sub inhibitory conc. (¾ MIC) of guava leaves extract showed lower relative density in protein bands at molecular weights of 70 KDa, 65 KDa , 60 KDa , 59 KDa , 49 KDa ,44 KDa& 36 KDa compared to the control.


Keywords: Pseudomonas aeruginosa, natural products/extracts, virulence factors, outer membrane proteins.
Download this article as Download

How to cite this article:

Ahmed Ahmed Abd El-Aziz, Tarek El-Said El-Banna, Fatma Ibrahim Sonbol and Shymaa Mohamed Sallam. 2017. Anti-Virulence Activity of Selected Natural Products against Pseudomonas aeruginosa.Int.J.Curr.Microbiol.App.Sci. 6(1): 396-410. doi: http://dx.doi.org/10.20546/ijcmas.2017.601.048