Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 2418-2427
DOI: https://doi.org/10.20546/ijcmas.2019.805.286


Genetic Diversity Analysis Based on SSR Markers in Daffodils (Narcissus)
S.I. Rehman1, M.Q. Sheikh1, Z.A. Bhat1 and M.H. Khan2*
1Division of Floriculture, S.K. University of Agricultural Sciences and Technology of Kashmir, Shalimar Srinagar (J&K), India
2Division of Genetics and Plant Breeding, S.K. University of Agricultural Sciences and Technology of Kashmir, Shalimar Srinagar (J&K), India
*Corresponding author
Abstract:

Daffodil (Narcissus spp.) belongs to family Amaryllidaceae and is a bulbous perennial grown for attractive flower, borne in spring sometimes autumn or winter. The present study was carried out to assess the genetic diversity present in daffodils in temperate region of Kashmir India using eleven sample sequence repeats (SSR’s). Twenty seven genotypes of daffodils belonging to different species such as Narcissus incomparabilis, Narcissus pseudonarcissus, Narcissus jonquilla, Narcissus poeticus, Narcissus papyraceus were evaluated for molecular characterization. Microsatellite markers revealed a high level of polymorphism and Jaccord’s Similarity coefficient ranged from 0.05 to 0.98. Analysis of molecular variance (AMOVA) revealed high level of variability of 94.89 per cent within population. Whereas among population variability is 5.11 per cent. The expected heterozygosity was shown highest (0.73) by marker A131 in Narcissus incomparibilis and total heterozygosity across different specieswas shown highest (0.71) by marker A5. The mean expected heterozygosity was shown highest (0.43) by Narcissus incomparabilis followed by Narcissus pseudonarcissus which revealed mean expected hytrozygosity of 0.37. The locus A109 and B112 revealed highest effective alleles of 10 each, followed by A5 and B104 recording effective alleles of 9 and 8 respectively.


Keywords: Narcissus spp., SSR, heterozygosity, polymorphic loci
Download this article as Download

How to cite this article:

Rehman, S.I., M.Q. Sheikh, Z.A. Bhat and Khan, M.H. 2017. Genetic Diversity Analysis Based on SSR Markers in Daffodils (Narcissus).Int.J.Curr.Microbiol.App.Sci. 8(5): 2418-2427. doi: https://doi.org/10.20546/ijcmas.2019.805.286