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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 2213-2222
DOI: https://doi.org/10.20546/ijcmas.2019.805.261


Phenotypic Detection of MRSA and Inducible Clindamycin Resistance among Clinical Isolates of Staphylococcus-Study done in a Tertiary Level Hospital
Ravindra S. Rathore1, Usha Verma1, Shahbaz Ali Khan1, Ekta Gupta2, Eshank Gupta2 and Prabhu Prakash1*
1Microbiology, Dr. S.N. Medical College, Jodhpur, India
2AIIMS, Jodhpur, India
*Corresponding author
Abstract:

Staphylococcus aureus is a versatile human pathogen causing infections ranging from relatively mild skin and soft tissue infection to life threatening sepsis, pneumonia and toxic shock syndrome. The increasing incidence of a variety of infections and, especially, the expanding role of community-associated methicillin-resistant S. aureus (MRSA)--has led to emphasis on the need for safe and effective agents to treat both systemic and localized staphylococcal infections. Clindamycin is considered to be one of the alternative agents in these infections. The present study was aimed to detect prevalence of inducible clindamycin resistance among S. aureus isolates and to study the relationship between clindamycin and methicillin resistance and correlation with multidrug resistance. During a period of6 months, a total of 245 Staphylococcal isolates from various clinical specimens were included in the study. Antimicrobial susceptibility test was done by Kirby-Bauer’s disc diffusion method, MRSA detection was done by using Cefoxitin discs. For detection of inducible clindamycin resistance, D test was done by double disc synergy (DDS) test using erythromycin and clindamycin antibiotic discs and three different phenotypes were interpreted as methicillin-sensitive (MS) phenotype (D test negative), inducible MLSB (iMLSB) phenotype (D test positive), and constitutive MLSB phenotype. In study time of 6 months 245 non repeated isolates of Staphylococci were detected from various clinical samples like blood, urine, pus, vaginal swab and other samples,157 were from Paediatrics and 33 from gynae. IPD and 55 were from OPD, 148 were S. aureus and 97 were CONS. Out of all 126 isolates were MRSA and 119 were MSS. In blood culture maximum MRSA were detected 86 out of 142(60.56%) Staphylococcal isolates. Vancomycin (5.30%) and Linezolid (6.93%) showed minimum resistance while Ciprofloxacin showed highest resistance 67.75%) Inducible Clindamycin resistance was seen in 40(16.32%) isolates,92 (37.55%) were having constitutional MLSB phenotype and 58 (23.67%) were MS Phenotype Laboratories where Vitek2 and molecular facilities are not available phenotypic detection of MRSA and inducible Clindamycin detection using DDS in all Staphylococcal isolates can be cost effective laboratory methodology and can guide in judicious use of antibiotics.


Keywords: Clindamycin, Resistance, MRSA, Staphylococcus
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How to cite this article:

Ravindra S. Rathore, Usha Verma, Shahbaz Ali Khan, Ekta Gupta, Eshank Gupta and Prabhu Prakash. 2019. Phenotypic Detection of MRSA and Inducible Clindamycin Resistance among Clinical Isolates of Staphylococcus-Study done in a Tertiary Level Hospital.Int.J.Curr.Microbiol.App.Sci. 8(5): 2213-2222. doi: https://doi.org/10.20546/ijcmas.2019.805.261