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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 2058-2068
DOI: https://doi.org/10.20546/ijcmas.2019.805.239


Characterization and Screening of Native Isolates of PSB and Azotobacter under in vitro Conditions
Hemlata Painkra, Tapas Chowdhury* and Narayan Prasad Verma
Department of Agricultural Microbiology, IGKV, Raipur-492006, Raipur, Chhattishgarh, India
*Corresponding author
Abstract:

The present study was conducted at Department of Agriculture Microbiology, College of Agriculture, IGKV, Raipur, C.G. during the year 2018-19 to characterize and screen different native isolates of PSB and Azotobacter. 14 microbial isolates were biochemically characterized and screened under in-vitro conditions for their plant growth promoting properties. Among 14 tested isolates Azoto-B-44, Azoto-146, Azoto-B-126, PSB-S-88, PSB-H-27, PSB-S-170, PSB-S-71, PSB-H-5, PSB-S-162 were shown positive results for TSI and Citrate test. PSB-172 and PSB-S-64 were shown positive for MR test and PSB-S-88, PSB-S-71, PSB-H-5, PSB-S-165 and PSB-S-162 were found positive for Gelatin liquefaction test. Rest of all isolates was negative towards above tests. All the isolates were taken for their antibiotic susceptibility study. Some isolates were found susceptible for Tetracycline (30mcg) and streptomycin (10mcg). In N-fixation study of Azotobacter isolates Azoto-B-126 found higher N-fixer, it fixed 3.25mg N/gm of sucrose. Azoto-123, Azoto-146 and Azoto-B-126 found significantly superior and at par for N-fixing capacity over Azoto-B-44. Ten isolates of PSB were screened for their P-solubilizing capacity. PSB-H-5 was found highest P-solubilizer (894.51 µg/ml), however all the isolates found significantly superior for P-solubilizing capacity over control. All the PSB isolates were also tested for their solubilizing efficiency of phosphorus in the form of solubilization zone. PSB-H-27 was found highest solubilization efficiency with solubilization zone of 14 mm diameter, however it found at par with PSB-S-162, PSB-S-165, PSB-S-71, PSB-H-5 and PSB-S-170.


Keywords: PSB, Azotobacter, BNF, Phosphorus solubilizing capacity.
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How to cite this article:

Hemlata Painkra, Tapas Chowdhury and Narayan Prasad Verma. 2019. Characterization and Screening of Native Isolates of PSB and Azotobacter under In-vitro Conditions.Int.J.Curr.Microbiol.App.Sci. 8(5): 2058-2068. doi: https://doi.org/10.20546/ijcmas.2019.805.239