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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 2010-2017
DOI: https://doi.org/10.20546/ijcmas.2019.805.233


Virulence Profiling of Listeria monocytogenes Isolated from Different Sources
S.R. Thorat1, D.B. Rawool4, P.M. Sonkusale1, S.R. Warke2, S.P. Choudhari3 and N.V. Kurkure1*
1Department of Veterinary Pathology,
2Department of Veterinary Microbiology,
3Veterinary Public Health, Nagpur Veterinary College, Nagpur-440006 and
4Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar 243 122, India
*Corresponding author
Abstract:

T A total of 23 putative Listeria isolates obtained from different sources, viz. food, animal, human, caterpillar and mosquito were screened for presence of the virulence factors by multiplex polymerase chain reaction (mPCR). Multiplex polymerase chain reaction for the amplification of isp and prs genes was employed for genus and species identification, while virulence profiling was employed by amplification of plcA, hlyA, actA, prfA, inlC, inlJ, luxS and fla genes. All strains harbours virulence genes plcA, hlyA, actA, prfA, inlC, inlJ, luxS and fla. Finally this study validated mPCR in the analysis and rapid detection and virulence profiling of L. monocytogenes. Irrespective of species of origin all the virulence genes are expressed by all isolates ‎coequally.


Keywords: Listeria monocytogenes, Virulence profiling, Multiplex-PCR
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How to cite this article:

Thorat, S.R., D.B. Rawool, P.M. Sonkusale, S.R. Warke, S.P. Choudhari and Kurkure, N.V. 2019. Virulence Profiling of Listeria Monocytogenes Isolated from Different Sources.Int.J.Curr.Microbiol.App.Sci. 8(5): 2010-2017. doi: https://doi.org/10.20546/ijcmas.2019.805.233