International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:5, May, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 2010-2017

Virulence Profiling of Listeria monocytogenes Isolated from Different Sources
S.R. Thorat1, D.B. Rawool4, P.M. Sonkusale1, S.R. Warke2, S.P. Choudhari3 and N.V. Kurkure1*
1Department of Veterinary Pathology,
2Department of Veterinary Microbiology,
3Veterinary Public Health, Nagpur Veterinary College, Nagpur-440006 and
4Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar 243 122, India
*Corresponding author

T A total of 23 putative Listeria isolates obtained from different sources, viz. food, animal, human, caterpillar and mosquito were screened for presence of the virulence factors by multiplex polymerase chain reaction (mPCR). Multiplex polymerase chain reaction for the amplification of isp and prs genes was employed for genus and species identification, while virulence profiling was employed by amplification of plcA, hlyA, actA, prfA, inlC, inlJ, luxS and fla genes. All strains harbours virulence genes plcA, hlyA, actA, prfA, inlC, inlJ, luxS and fla. Finally this study validated mPCR in the analysis and rapid detection and virulence profiling of L. monocytogenes. Irrespective of species of origin all the virulence genes are expressed by all isolates ‎coequally.

Keywords: Listeria monocytogenes, Virulence profiling, Multiplex-PCR

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How to cite this article:

Thorat, S.R., D.B. Rawool, P.M. Sonkusale, S.R. Warke, S.P. Choudhari and Kurkure, N.V. 2019. Virulence Profiling of Listeria Monocytogenes Isolated from Different Sources.Int.J.Curr.Microbiol.App.Sci. 8(5): 2010-2017. doi:
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