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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 1634-1649
DOI: https://doi.org/10.20546/ijcmas.2019.805.189


Evaluation of Quorum Quenching and Probiotic Activity of Bacillus thuringiensis QQ17 Isolated from Fish Culture Pond
Divya V. Haridas1,2* and Devika Pillai1
1Kerala University of Fisheries and Ocean Studies, Department of Aquatic Animal Health Management,  Centre for Aquatic Animal Health, Panangad P.O., Kochi, Kerala, India, Pin- 682 506
2Mahatma Gandhi University, School of Biosciences, Kottayam, Kerala, India, Pin- 686 560
*Corresponding author
Abstract:

This work was aimed at isolating AHL degrading bacteria from fish culture pond soil, with abilities appropriate for use as probiotic in aquaculture. The presence of an autoinducer inactivation (aiiA) homologue gene and AHL-inactivation assay showed that BacillusthuringiensisQQ17, which was one among the 20 isolates, could rapidly degrade synthetic C6-HSL in vitro and hampered violacein production by Chromobacterium violaceum. It had excellent biodegrading ability of natural N-AHL produced by Aeromonas hydrophila, suggesting that it can be used as a potential quencher bacterium for inhibiting the virulence of A. hydrophila. The isolate grew well at pH 3.0-7.0, was resistant to high level of bile salts (0-0.9%) and 0.5 % of phenol. QQ17 also exhibited high degree of auto-aggregation and co-aggregation, confirming that it possessed good probiotic attributes. It was susceptible to all the 11 antibiotics tested and exhibited antagonistic activity against A. hydrophila. Gold fish fed diet incorporated with 108 and 1010 CFU/g of the QQ17 for 30 days showed 73.33-83.33% survival when challenged with pathogenic A. hydrophila. The study indicates that the isolate B. thuringiensis QQ17 could be used as a non- antibiotic feed additive in aquaculture to control bacterial diseases.


Keywords: Quorum sensing, Quorum quenching, N-acyl-homoserine lactones, Probiotic, Bacillus thuringiensis
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How to cite this article:

Divya V. Haridas and Devika Pillai. 2019. Evaluation of Quorum Quenching and Probiotic Activity of Bacillus thuringiensis QQ17 Isolated from Fish Culture Pond.Int.J.Curr.Microbiol.App.Sci. 8(5): 1634-1649. doi: https://doi.org/10.20546/ijcmas.2019.805.189