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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 1564-1572
DOI: https://doi.org/10.20546/ijcmas.2019.805.181


Comparative Study of Lipopolysaccharide and Killed Vaccines of Brucella suis Field Isolate in Mice
N.R. Senthil1*, M. Vijaya Bharathi2, G. Selvaraju3, S. Manoharan4 and K.G. Tirumurugan5
1Centralised Clinical Laboratory, Madras Veterinary College, Chennai, India
2Research Institute for Animal Sciences, Kancheepuram, India
3Department of Veterinary Public Health and Epidemiology, VC&RI, Namakkal, India
4 Bacterial Vaccines, CAHS, Madhavaram Milk Colony, Chennai, India
5Animal Biotechnology, Madras Veterinary College, Chennai, India
*Corresponding author
Abstract:

In the present study, we developed a killed and LPS based Brucella suis vaccine and evaluated the humoral and cell mediated immune response in mice. Brucella suis isolated from field and developed as vaccine. Lipopolysaccharides from B. suis were administered to the nude mice, in addition to killed vaccine from that species whereas mineral oil was used as an adjuvant. The vaccines from B. suis is considered as smooth strain with smooth dissociation. Serum was collected from clotted blood samples of day 0, 7, 14 and 21 from the mice (control, adjuvant, BM-killed, BM-LPS vaccine, BS-killed and BS-LPS groups). In BS-killed and BS-LPS treated groups, the immune response was very significant in BS-LPS group alone. Though, the immune response was lower in BS-LPS vaccine group (p value = 0.0075) in comparison to BS-killed vaccine group (p value = 0.0009), 14 days post vaccination. There is a significant spike on the immune response of the BS-LPS group (p value < 0.0001), 21 days post vaccination. In B. suis vaccinated groups, the TNFα, IFNγ, IL4, IL10 and IL12 expressions were up-regulated in BS-LPS treated animals with a p value of 0.0198, 0.0142, 0.0195, 0.0384 and 0.03 respectively.


Keywords: Brucella suis, Lipopolysaccharide, Humoral immunity and Cytokine expression
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How to cite this article:

Senthil, N.R., M. Vijaya Bharathi, G. Selvaraju, S. Manoharan and Tirumurugan, K.G. 2019. Comparative Study of Lipopolysaccharide and Killed Vaccines of Brucella suis Field Isolate in Mice.Int.J.Curr.Microbiol.App.Sci. 8(5): 1564-1572. doi: https://doi.org/10.20546/ijcmas.2019.805.181