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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 1457-1463
DOI: https://doi.org/10.20546/ijcmas.2019.805.167


Molecular Characterization and Sero-epidemiological Study of Leptospirosis in Cattle of Nagpur and Surrounding Regions
Shilpa L. Moon1*, Sandeep P. Chaudhari1, Nandkishor N. Zade1, Wiqar A. Khan1, Shilpshri V. Shinde1, Nitin V. Kurkure2, Sukhdeo B. Barbuddhe3, Aanusha Alamuri4 and V. Balamurugan4
1Department of Veterinary Public Health, Nagpur Veterinary College, MAFSU, Nagpur, Maharashtra, India
2Department of Veterinary Pathology, Nagpur Veterinary College, MAFSU, Nagpur, Maharashtra, India
3ICAR-National Research Centre on Meat (NRCM), Hyderabad, Telangana, India
4ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Bengaluru, Karnataka, India
*Corresponding author
Abstract:

A zoonotic disease Leptospirosis is caused by pathogen of the genus Leptospira and it is an emerging global public health problem. In the present study a total of 266 cattle blood and sera samples were collected from the towns and villages of Nagpur, Wardha, Bhandara Gadchiroli and Durg districts during December 2017 to 2018 and tested for the presence of Leptospira. These samples were from randomly selected herds with history of repeated breeding, abortion, reproductive disorders, etc. also including some apparently healthy animals. The presence of leptospiral DNA in blood sample was assayed by PCR amplification of rrs (16S rRNA) gene. Antibodies against Leptospira serovars were tested using an enzyme-linked immunosorbent assay (ELISA) and microscopic agglutination test (MAT). Out of 266 blood samples, in 33 samples leptospires DNA was identified with the frequency of 12.40%. A total of 53 cattle sera were positive in commercial Leptospira Bovine Hardjo ELISA kit indicated 19.92% seroprevalence. In the MAT analysis 120 samples revealed presence of different serovars with the seropositivity 45.11%. The study supports the probable role of cattle in maintaining Leptospira Hardjo along with some other serovars and warrants an intensive control and surveillance programme for reducing leptospirosis in cattle.


Keywords: Leptospirosis, Cattle, Nagpur, PCR, ELISA, MAT
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How to cite this article:

Shilpa L. Moon, Sandeep P. Chaudhari, Nandkishor N. Zade, Wiqar A. Khan, Shilpshri V. Shinde, Nitin V. Kurkure, Sukhdeo B. Barbuddhe, Aanusha Alamuri and Balamurugan, V. 2019. Molecular Characterization and Sero-epidemiological Study of Leptospirosis in Cattle of Nagpur and Surrounding Regions.Int.J.Curr.Microbiol.App.Sci. 8(5): 1457-1463. doi: https://doi.org/10.20546/ijcmas.2019.805.167