International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:5, May, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 1395-1411

Study of Genetic Polymorphism in Wheat and its Wild Relatives using ISSR Markers
Payal Saxena* and V.K. Khanna
Department of Genetics and Plant Breeding, College of Agriculture, G.B. Pant University of Agriculture and Technology, Pantnagar- 263145 (U.S. Nagar, Uttarakhand), India
*Corresponding author

Inter Simple Sequence Repeats, the PCR based technique was used to reveal the genetic diversity among wheat and its wild relatives. DNA profiling of 27 wheat genotypes at all the existing ploidy levels (diploid, tetraploid and hexaploid) was done using ISSR primers. All the primers were polymorphic. The primers resulted in the amplification of 73 bands out of which 17 bands were unique. Unique bands resulted in case of all the primers. According to these 4 ISSR primers genetic similarity value was observed ranging from 0 to 1, the highest genetic similarity value (1.00) was noticed between T. timopheevii and T. sphaerococcum followed by 0.909 between T. tauschii - T. timopheevii and T. tauschii -T. sphaerococcum. The lowest genetic similarity value (0.00) was noticed between PBN 51 and NIAW 34 owing to their different places of release. It was followed by 0.032 between PBW 373 and PDW 291 as one is hexaploid and the other one is tetraploid wheat.

Keywords: ISSR, Dendrogram, Wheat, Wild relatives of wheat

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How to cite this article:

Payal Saxena and Khanna, V.K. 2019. Study of Genetic Polymorphism in Wheat and its Wild Relatives using ISSR Markers.Int.J.Curr.Microbiol.App.Sci. 8(5): 1395-1411. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.