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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 1056-1067
DOI: https://doi.org/10.20546/ijcmas.2019.805.124


Molecular Characterization of Wheat (Triticum sp.) Genotypes Grown in Humid South Eastern Plain Zone of Rajasthan
Praveen Kumar Chachaiya1*, Krishnendra Singh Nama1 and Gargi Mehta2
1Department of Life Science, Career Point University, Kota, Rajasthan, India
2Department of Botany, Jiwaji University, Gwalior, Madhya Pradesh, India
*Corresponding author
Abstract:

Wheat is qualitatively a vital source of macromolecule, energy and fiber for human nutrition since decades hence, used as a staple food grain for community and as well a major source of fodder for animal feeding. Assessment of genetic diversity using molecular markers is used for characterization of different genotypes with reliable and authentic results. RAPD is a PCR based molecular technique for identification of genetic variability in similar genotypes. It usually preferred for the initiation of this kind of work as this technique is simple, versatile and relatively inexpensive. 35 amplified bands were obtained using 6 RAPD primers, in which (54.29%) were polymorphic and (45.71%) was monomorphic. Total amplified bands varied in between 5 to 7 with an average of 5.83 bands/primer. Average PIC value was 0.143 with ranging from 0.036 to 0.296. The lowest and the highest PIC value were recorded for primer OPA 14 and OPA 05, respectively. UPGMA cluster constructed from RAPD analysis clubbed the 10 wheat genotypes into three major clusters I, II and III and I was further divided in sub clusters IA and IB. The eager knowledge of genetic diversity is used to diagnose genetic programme and beneficial for future crop improvement.


Keywords: Wheat, Genotypes, Diversity, RAPD, Marker, DNA, UPGMA
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How to cite this article:

Praveen Kumar Chachaiya, Krishnendra Singh Nama and Gargi Mehta. 2019. Molecular Characterization of Wheat (Triticum sp.) Genotypes Grown in Humid South Eastern Plain Zone of RajasthanInt.J.Curr.Microbiol.App.Sci. 8(5): 1056-1067. doi: https://doi.org/10.20546/ijcmas.2019.805.124