International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:5, May, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 1003-1012

Efficient Callus Induction through Anther Culture in Cultivars of Brassica campestris var. Brown Sarson
Sheetal Sood* and Vedna Kumari
Department of Crop Improvement, CSK HP KV Palampur, Himachal Pradesh-176061, India
*Corresponding author

In the present study, anther of three varieties and their cross combinations of Brassica campestris, namely HPBS-1, KBS-3, HPKM-04-1, HPBS-1 x HPKM-04-1 and KBS-3 x HPKM-04-1 were cultured in vitro to observe their callus induction frequency. The effect of two basal media i.e. MS and N6, two different sucrose concentrations i.e. 3% and 4%, three combinations of growth hormones viz. HM1, HM2, HM3 and their callus induction frequency were analyzed by CPCS software. Out of all factors and their interactions, the genotype HPBS-1 performed better in N6 medium with HM2 [0.5 mg/l 2, 4-D + 1.0 mg/l NAA] and 3 per cent sucrose for callus induction frequency. This media and hormonal combination can be successfully utilized for induction of haploids and double haploids in future.

Keywords: Brassica campestris, Callus, Hormones, Media, Anther culture

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How to cite this article:

Sheetal Sood and Vedna Kumari. 2019. Efficient Callus Induction through Anther Culture in Cultivars of Brassica campestris var. Brown Sarson.Int.J.Curr.Microbiol.App.Sci. 8(5): 1003-1012. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.