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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 804-811
DOI: https://doi.org/10.20546/ijcmas.2019.805.095


Characterization of Excretory and Secretory Larval Antigen of Toxocara canis by Western Blotting
K.T. Kavitha2*, C. Sreekumar1, B.R. Latha2, A. Mangala Gowri3 and B. Nagarajan4
1Department of Wildlife Science, Madras Veterinary College, Chennai 600 007, India
2Department of Veterinary Parasitology, Madras Veterinary College, Chennai 600 007, India
3Stem Cell Research and Regenerative Medicine, Madras Veterinary College, Chennai 600 007, India
4Department of Veterinary Preventive Medicine, Madras Veterinary College,Chennai 600 007, India
*Corresponding author
Abstract:

Toxocara canis is a dog helminth which causes visceral larva migrans (VLM) in humans. The identification of specific antigens of T. canis is important in order to develop better diagnostic techniques. The excretory-secretory larval antigens of T. canis (ESLA) were prepared by in vitro culturing of T. canis larvae in RPMI 1640 medium. These antigens were separated using sodium dodecyl sulphate-poly acrylamide gel electrophoresis (SDS-PAGE) which revealed 9 protein bands at a molecular weight of 17, 18, 22, 24, 26, 28, 30, 32 and 120 kDa. The immuno reactivity of excretory-secretory larval antigens of T. canis was checked by Western blotting using hyper immune serum raised in rabbits against ESLA antigen which showed six immuno reactive bands at a molecular weight of 17, 18, 22, 24, 30 and 32 kDa. These antigens merit further evaluation as candidate for use in diagnosis of toxocariasis in humans and adult dogs.


Keywords: Toxocara canis, Excretory-secretory larval antigens, Western blotting
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How to cite this article:

Kavitha, K.T., C. Sreekumar, B.R. Latha, A. Mangala Gowri and Nagarajan, B. 2019. Characterization of Excretory and Secretory Larval Antigen of Toxocara canis by Western Blotting.Int.J.Curr.Microbiol.App.Sci. 8(5): 804-811. doi: https://doi.org/10.20546/ijcmas.2019.805.095