International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:5, May, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 588-596

Effect of Extenders Containing Glycerol and Egg Yolk on Motility and Viability of Chilled Ram Semen collected during Non-Breeding Season
S. Yotov1*, M. Karadaev1, A. Atanasov1, I. Fasulkov1, A. Antonov1 and E. Kistanova2
1Department of Obstetrics, Reproduction and Reproductive Disorders, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria
2Bulgarian Academy of Sciences, Institute of Biology and Immunology of Reproduction, 1113 Sofia, Bulgaria
*Corresponding author

The aim of this study was to evaluate the effect of Tris-based extenders containing glycerol and egg yolk on the motility and viability of chilled ram semen collected during non-breeding season. Nine ejaculates from three healthy rams in non-breeding season were collected by artificial vagina method. The semen was diluted with Steridyl (ST), Triladyl (TRY) or Tris-based extender containing 5 % glycerol and 5% egg-yolk (TGGY) to a final concentration 200x109 spermper ml. Semen samples (n=9) for each extender were stored at 50C for 72 hours. Motility and viability were evaluated at 0, 6, 24, 48 and 72 h of storage. The results were statistically processed and the influence of extender and time of storage were recorded. After 6 h of storage the initial motility and viability did decrease (P<0.05) in all extenders but until 48 h significant differences among values for the same interval were not determined. At 72 h the values for ST and TGGY were increased (P<0.05) compared to TRY, but rather unsatisfactory. The increased time of storage was negatively correlated with both indicators in all extenders (P<0.05). In conclusion, Tris-based extenders containing glycerol and egg yolk demonstrated good protective effect on ram semen collected during the non-breeding season and stored at 50C. The type of extender had no influence on sperm motility and viability until 48 h of storage, while the time of storage significantly (P<0.05) affected the semen parameters.

Keywords: Ram, Chilled semen, Extender, Glycerol, Egg yolk, Time of storage

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How to cite this article:

Yotov, S., M. Karadaev, A. Atanasov, I. Fasulkov, A. Antonov and Kistanova, E. 2019. Effect of Extenders containing Glycerol and Egg Yolk On Motility and Viability of Chilled Ram Semen collected during Non-Breeding Season.Int.J.Curr.Microbiol.App.Sci. 8(5): 588-596. doi:
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