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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(1): 143-154
DOI: http://dx.doi.org/10.20546/ijcmas.2017.601.018


Production, Extraction and Characterization of Red Pigment Produced by Serratia rubidaea JCM 1240T isolated from Soil
R.R. Rakh1*, S.M. Dalvi2, B.B. Musle3 and L.S. Raut4
1Department of Microbiology, Shri Guru Buddhiswami Mahavidyalaya, Purna (Jn.) Dist. Parbhani, India
2Department of Botany, Shri Guru Buddhiswami Mahavidyalaya,Purna (Jn.) Dist. Parbhani, India
3Department of Chemistry, Shri Guru Buddhiswami Mahavidyalaya,Purna (Jn.) Dist. Parbhani, India
4Department of Microbiology, Sant Tukaram College, Parbhani, Dist. Parbhani, India
*Corresponding author
Abstract:

A red pigment producing bacteria was isolated from soil sample which was identified by 16S r RNA sequencing as Serratia rubidaea JCM 1204T. The red pigment produced was tested for prodigiosin by presumptive color test, adsorption spectra, and its quantification was carried out on King’s B medium. For presumptive color test, the extracted pigment solution was divided into two portions. One part was acidified with a drop of concentrated HCl and the other part was alkalinized with a drop of concentrated ammonia solution. A red or pink color in the acidified solution and a yellow or tan color in the alkaline solution indicated a positive, presumptive test for prodigiosin. For adsorption spectra, the ethanolic acidified and alkalinized red pigment extract was tested by spectrophotometer in range between 300 to 700 nm and found that the ethanolic acidified red pigment extract absorbed maximum light at 535 nm while ethanolic alkalinized red pigment extract absorbed maximum light at 470 nm which is characteristic of prodigiosin. The solid red pigment was also further characterized by IR, NMR, and Mass spectrophotometer, which confirms that the red pigment produced by Serratia rubidaea JCM 1204T as prodigiosin.


Keywords: Serratia rubidaea JCM 1204T, Prodigiosin, Presumptive color test, IR, NMR and Mass spectrophotometer.
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How to cite this article:

Rakh, R.R., S.M. Dalvi, B.B. Musle and Raut, L.S. 2017. Production, Extraction and Characterization of Red Pigment Produced by Serratia rubidaea JCM 1240T isolated from Soil.Int.J.Curr.Microbiol.App.Sci. 6(1): 143-154. doi: http://dx.doi.org/10.20546/ijcmas.2017.601.018