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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(5): 296-312
DOI: https://doi.org/10.20546/ijcmas.2019.805.035


Screening and Isolation of Lipase Producing Bacteria from Contaminated Soils from the Littoral-Region of Cameroon and Partial Study of the Fermentation Conditions of the Crude Enzyme Produced
Fobasso Tagnikeu Romeo1, Tavea Fréderic Marie1*, Tetso Ghislain Brice3, Tchamba Mbiada Mervie Noel4, Tcheugoue Styve Joel1, Momo Gautierand Etoa François Xavier2
1Department of Biochemistry, Faculty of Science, University of Douala, Cameroon
2Department of Biochemistry, Faculty of Science, University of Yaoundé I, Cameroon
3Department of Biochemistry, Faculty of Science, University of Buea, Cameroon
4Department of Food Sciences and Nutrition, ENSAI Ngaoundéré, Cameroon
*Corresponding author
Abstract:

Lipases are enzymes that catalyze the transformation reactions of triglycerides in to fatty acids and glycerol. They can be produced by animals, plants and microorganisms. This article presents the isolation of lipase-producing bacteria from soil samples collected from sites contaminated with waste from palm oil production in the littoral region of Cameroon. These permit to isolate a multitude of bacteria capable of producing lipase from Rhodamine B-agar olive oil culture medium. Of the 35 isolates obtained from the 66 soil samples, one was selected as the best based on its enzymatic activity, the DI1A isolate. After production of the crude enzyme, the influence of temperature and pH on it was studied, followed by the impact of some physicochemical parameters on the fermentation medium. The DI1A isolate produced a crude enzyme showing better activity of 5.63±0.31 IU/ml with optimal activity at a temperature of 55°C and a pH of 8. The study of the influence of some physico-chemical parameters on the isolate showed an optimal growth temperature at 38°C, an optimum pH at 7, the best carbon source is palm kernel oil at 1% (v/v), the best nitrogen source is ammonium chloride at 2% (m/v) and the best salt source is magnesium sulphate at 3% (m/v). Macroscopic and microscopic analyses reveal that the DI1A isolate is a Gram+ bacillus, a positive catalase capable of sporulating.


Keywords: Thermostable lipase, Isolation, Littoral-Cameroon, Fermentation, Bacillus
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How to cite this article:

Fobasso Tagnikeu Romeo, Tavea Fréderic Marie, Tetso Ghislain Brice, Tchamba Mbiada Mervie Noel, Tcheugoue Styve Joel, Momo Gautier and Etoa François Xavier. 2019. Screening and Isolation of Lipase Producing Bacteria from Contaminated Soils from the Littoral-Region of Cameroon and Partial Study of the Fermentation Conditions of the Crude Enzyme Produced.Int.J.Curr.Microbiol.App.Sci. 8(5): 296-312. doi: https://doi.org/10.20546/ijcmas.2019.805.035