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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(1): 100-107
DOI: http://dx.doi.org/10.20546/ijcmas.2017.601.013


Increased ROS Generation: Implication in Antibacterial Activity of Evolvulus nummularius against Multidrug Resistant Gram Negative Bacterial Strains

Susmita Saha1, Anindya Das1, Angkita Debnath1, Shipa Begam1, Subrata Sen1, Swapan Majumdar2 and Samir Kumar Sil1*

1Molecular Genetics and Cell Physiology Lab., Department of Human Physiology, Tripura University, Suryamaninagar, West Tripura-799022, India.
2Department of Chemistry, Tripura University, Suryamaninagar, West Tripura-799022, India
*Corresponding author
Abstract:

Evolvulus nummularius, is ethnomedicinally used as a medicine for hysteria, to cure burns, cuts, wounds and scropion stings. This plant has antihelminthic activity, wound healing activity, poor sedative and anticonvulsant properties. In this study, the antibacterial activity of crude methanol extract of E. nummularius was studied against three gram negative multidrug resistant strains E. aerogenes, P. aeruginosa and K. pneumoniae. This plant extract showed both bacteriostatic and bactericidal activity against all these bacterial strains. The extract was most active against E. aerogenes.  In growth analysis study, it was obtained that, after treatment with IC50 dose for each bacterial strains, the lag phase become extended compared to untreated bacterial cells. To understand the mechanism of action of Evolvulus nummularius the reactive oxygen species (ROS) was estimated and result showed that, reactive oxygen species were increased (~ 35-60%) in presence of IC50 dose of Evolvulus nummularius.


Keywords: Evolvulus nummularius, Gram negative bacteria, MDR strains, Growth curve, ROS.
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How to cite this article:

Susmita Saha, Anindya Das, Angkita Debnath, Shipa Begam, Subrata Sen, Swapan Majumdar and Samir Kumar Sil. 2017. Increased ROS Generation: Implication in Antibacterial Activity of Evolvulus nummularius against Multidrug Resistant Gram Negative Bacterial Strains.Int.J.Curr.Microbiol.App.Sci. 6(1): 100-107. doi: http://dx.doi.org/10.20546/ijcmas.2017.601.013